Tissue-engineered skeletal muscle for myokine secretion

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H04520
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Osaka Institute of Technology
• Principal Investigator: FUJISATO Toshiya 大阪工業大学, 工学部, 教授 (60270732)
• Co-Investigator(Kenkyū-buntansha): 中村 友浩 大阪工業大学, 工学部, 教授 (30217872) ; 横山 奨 大阪工業大学, 工学部, 講師 (30760425)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 組織工学 / 培養骨格筋 / マイオカイン / IL-6 / 培養肉

Outline of Final Research Achievements

Myokines secreted during skeletal muscle movement have attracted much attention. Currently, there are more than several dozen myokines, many of which are unknown. In the in vivo search for myokines using living animals, it is not easy to clearly evaluate myokines due to the influence of other tissues than skeletal muscle. Using cultured skeletal myoblasts and collagen, we developed a new device to evaluate myokines secreted under various contraction modes, such as isometric and isotonic, in order to identify myokines and optimize the secretion conditions. As a result, we confirmed that the highest contractility and secretion of IL-6 were observed under the condition of 6 hours of electrical stimulation per day for 7 days.

Free Research Field

生体組織工学

Academic Significance and Societal Importance of the Research Achievements

培養骨格筋に各種電気刺激を与え、収縮力およびマイオカインIL-6の分泌について検討した。無数にある電気刺激条件の中で、培養骨格筋の収縮力が低下せず、また培地のpH等への影響も少ない周波数0.5Hzに固定して検討したところ、1日あたり6時間の電気刺激を7日間加えた条件が、最も収縮力およびIL-6の分泌が高いことを確認した。また、培養骨格筋周囲をアルギン酸ゲル膜でコーティングすることで、培養骨格筋内に分泌されたIL-6を閉じ込めることを確認した。これによって、マイオカインを豊富に含む培養食肉への応用可能性が示唆された。