The molecular mechanism of skeletal muscle atrophy by analyzing chromatin structure in skeletal muscle

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K08634
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Osaka University
• Principal Investigator: Inoue Kazunori 大阪大学, 大学院医学系研究科, 助教 (10631301)
• Co-Investigator(Kenkyū-buntansha): 安田 聖一 大阪大学, 医学部附属病院, 医員 (00869927) ; 島田 果林 大阪大学, 医学部附属病院, 医員 (60814770)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 慢性腎臓病 / ビタミンD / 骨格筋

Outline of Final Research Achievements

We have measured skeletal muscle weight (slow-twitch soleus and fast-twitch extensor digitorum longus) in control and vitamin D receptor knockout (Vdr KO) mice, and found skeletal muscle weight decreased significantly in Vdr KO mice compared to control mice at the age of 3 months. Next, we performed RNAseq in the mice skeletal muscles to analyze mRNA expression comprehensively. Also, we examined RNAseq in the skeletal muscles of 5/6 nephrectomized mice in a similar way as Vdr KO mice. Differentially expressed genes (DEGs) were found in both RNAseq data and the common genes of the DEGs were identified.We are examining how these genes were regulated, especially by transcription factors.

Free Research Field

腎臓病学

Academic Significance and Societal Importance of the Research Achievements

高齢化社会を迎え、慢性腎臓病患者も高齢化しており、筋力低下による転倒、骨折が医療的、医療経済的な問題となっている。本研究により慢性腎臓病の進展による骨格筋萎縮の分子機序が明らかとなれば、これまで候補治療薬とされてきた活性型ビタミンDなどと併用もしくはそれらに代わる新規治療薬創生の分子基盤となり得る。