2023 Fiscal Year Final Research Report

development of liver cancer therapy using polyamide targeting tgf--b1

Research Project

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Project/Area Number	20K09089
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55020:Digestive surgery-related
Research Institution	Nihon University
Principal Investigator	TAKAGI Keiko 日本大学, 医学部, 研究医員 (20339328)
Co-Investigator(Kenkyū-buntansha)	緑川泰国立研究開発法人国立精神・神経医療研究センター, 病院総合外科部, 部長 (10292905) 阿部勇人日本大学, 医学部, 助教 (10838478) 福田昇日本大学, 医学部, 教授 (40267050)
Project Period (FY)	2020-04-01 – 2024-03-31
Keywords	肝癌細胞 / TGF-β / PIポリアミド / 肝癌
Outline of Final Research Achievements	In experiments on rat liver cancer cells, no significant difference was observed between the concentration of PI polyamide and the expression level of TGF-β after administration of TGF-β1-inhibitory PI polyamide. However, in experiments on human liver cancer cells, the use of certain PI polyamides dose-dependently decreased TGF-β1 mRNA levels in HepG2 and HLF cells and inhibited HepG2 colony formation. Although PI polyamide did not substantially inhibit the proliferation of HepG2 cells compared to untreated control cells, it significantly inhibited the invasion of HLF cells, also inhibited the proliferation of HLE and HLF cells, and inhibited the formation of HLF cell spheres formation was significantly inhibited.
Free Research Field	肝臓癌
Academic Significance and Societal Importance of the Research Achievements	本研究は、肝癌細胞株に対するTGF-β1抑制性PIポリアミドの機能解析を行い、肝癌治療法の開発を目的としている。ヒトTGF-β1 プロモーターを標的とする PI ポリアミド投与後の TGF-β1 発現量を解析し、細胞増殖、浸潤性、TGF-β1 mRNA 発現量に及ぼす影響を検討した。TGF-β1抑制性PIポリアミドは、肝癌細胞における TGF-β1 発現を減少させ、細胞浸潤を抑制した。このことからTGF-β1抑制性PIポリアミドは肝臓癌治療の新規候補薬となる可能性があると考えられた。