Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K09431
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Kanazawa University
• Principal Investigator: Kato Satoshi 金沢大学, 附属病院, 助教 (30584841)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 転移性骨腫瘍 / 凍結療法 / 免疫増強

Outline of Final Research Achievements

We investigated the abscopal effect after cryoablation (CA) on bone metastasis using a mouse model. Breast cancer cells were implanted in the bilateral tibiae of mice. The left tumor was treated locally with CA, and the right abscopal tumor (AT) was left untreated. The mice were divided into four groups based on the combination of CA and intraperitoneal administration of anti-PD-1 antibody (PD) as treatment interventions (Control, CA, PD, and CA + PD). The reduction ratio of the size of AT, the quantitative immune effects at enzyme-linked immunospot (ELISPOT) assay, and the intensity of infiltration of immune-related cells to AT were compared among the groups. The results suggested that the abscopal effect associated with the local cryotherapy of metastatic bone tumors was activated by CA and enhanced by its combination with PD.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

本研究より、Cryoablationのがん特異的免疫効果と抗PD-1抗体による増強を示した。この結果は骨転移に対する局所治療の意義をさらに高める。骨関連事象に対する手術にCryoablation併用することで、腫瘍の局所制御効果の増強に他の遠隔転移巣に対するがん免疫効果の増強が付加されるHybrid surgeryの開発を目指している。特に、Performance Status低下や手術介入の機会が多い脊椎転移の患者にとっては本研究が目指すHybrid surgery恩恵は大きい。今後はがん免疫上昇効果におけるCryoablationの有利性について他の局所療法を比較検討する予定である。