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2022 Fiscal Year Final Research Report

Study on circulating tumor cell capture of genitourinary cancer by novel fluidic chip device

Research Project

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Project/Area Number 20K09588
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56030:Urology-related
Research InstitutionNippon Medical School

Principal Investigator

Kondo Yukihiro  日本医科大学, 大学院医学研究科, 大学院教授 (80215467)

Co-Investigator(Kenkyū-buntansha) 木村 剛  日本医科大学, 医学部, 教授 (20234354)
大林 康太郎  日本医科大学, 医学部, 助教 (30857579)
鈴木 康友  日本医科大学, 医学部, 准教授 (90297911)
Project Period (FY) 2020-04-01 – 2023-03-31
Keywordsgenitourinary cancer / circulating tumor cell / fluidic chip device
Outline of Final Research Achievements

Confirmation of antigen expression and cell capture experiments in prostate cancer cell lines (PC3 and LNCaP) were performed using a novel fluidic chip device. The capture rate was 83.82% for PC3 and 75.78% for LNCaP, demonstrating a high capture rate in prostate cancer cell lines. In addition, 2 mL of peripheral blood was collected from a patient with untreated metastatic prostate cancer, passed through the chip, and successfully captured CTCs. The captured CTCs were observed under a fluorescence microscope after being subjected to various fluorescent immunostaining. Under this condition, urothelial tumors and renal cell carcinomas were similarly examined using cultured microparticles, but the capture rate was lower than that of prostate cancer. Reflecting this, 2 mL of blood could not be sufficiently captured even in clinical specimens.

Free Research Field

尿路生殖器癌

Academic Significance and Societal Importance of the Research Achievements

本新規流体ディバイスはとても簡易システムであり安価である。また循環腫瘍細胞は癌治療に関してのマーカーになりうるものである。前立腺癌においてはPSAという腫瘍マーカーが存在するがその他の尿路上皮癌や腎癌では、明確な腫瘍マーカーが存在します。その点で本ディバイスを用いることにより新規腫瘍マーカーとなり、捕捉した細胞を解析することにより治療手段を選択可能にするものである。よって簡易で安価なこのディバイスが、広く臨床に応用される期待が高いものである。

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Published: 2024-01-30  

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