Investigation of Microbiota and Macrophage as Host Factors in the Pathogenesis of Chronic

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17237
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: Ikegami Hiroaki 産業医科大学, 医学部, 非常勤医師 (30833511)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 慢性肺アスペルギルス症

Outline of Final Research Achievements

Background; Chronic pulmonary aspergillosis is a refractory respiratory infection. In chronic pulmonary aspergillosis, the involvement of the normal bacterial flora in the pathogenesis of lung lesions has not yet been elucidated. Methods; The study targeted 24 cases suspected of chronic pulmonary aspergillosis, who underwent bronchoscopy at the Hospital of the University of Occupational and Environmental Health, Japan. A method combining the Sanger method with a clone library method was used for bacterial flora analysis. Results; Among the 15 cases diagnosed with chronic pulmonary aspergillosis, PCR positivity in the 16S rRNA region was observed in 11 cases, with genus Streptococcus (9 cases) and anaerobic bacteria (10 cases) being frequently detected. Conclusion; Mixed infection of the genus Streptococcus and anaerobic bacteria may be implicated in the pathogenesis of chronic pulmonary aspergillosis.

Free Research Field

細菌叢

Academic Significance and Societal Importance of the Research Achievements

これまでに慢性肺アスペルギルス症に対しては、背景病態として、嫌気性菌などの一般細菌が関与していることを検討している報告はなく、一般細菌が本症の病態や進行に関与していることが明らかになれば、治療に難渋することが多い慢性肺アスペルギルス症に対する有効な治療戦略に対する新しい知見が見いだされる可能性が考えられ、社会に大きく貢献できることが期待される。今回の解析からは慢性肺アスペルギルス症においてStreptococcus属と嫌気性菌の混合感染が多い傾向を認め、病態や宿主因子、疾患進行との関係性について今後さらなる検討が必要である。