Clarification of pathophysiology of peri-implantitis based on macrophages and osteocyte autophagy

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18608
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Nagasaki University
• Principal Investigator: INABA Nao 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (00814170)
• Project Period (FY): 2021-02-01 – 2024-03-31
• Keywords: インプラント周囲炎 / リポ多糖 / 炎症 / 慢性疾患

Outline of Final Research Achievements

The aim of this study was to clarify the pathophysiology of peri-implantitis based on autophagy by development of a rat model of lipopolysaccharide-induced peri-implantitis-like lesions. A rat model of peri-implantitis-like lesions was created and comprehensive analyses were performed. As results of quantitative analyses, collagen production and the number of blood vessels were significantly reduced in peri-implantitis-like lesions when compared to control group (normal tissue around implants). The distribution of 4 macrophage subtypes and several subtypes of T cells were significantly changed in peri-implantitis-like lesions when compared to control group. Moreover, the expression of autophagy in macrophage subtype A and T cell subtype X was significantly altered in peri-implantitis-like lesions when compared to control group. Our findings suggest us that autophagy is associated with the pathophysiology of peri-implantitis.

Free Research Field

補綴系歯学

Academic Significance and Societal Importance of the Research Achievements

インプラント周囲炎は国民病と呼ばれる歯周病と同様に，決定的治療法がない難治性慢性疾患で，原因も分かっていない．今回の研究では，インプラント周囲炎モデルラットを作製して詳細な解析を行い，インプラント周囲炎では，マクロファージとT細胞という免疫細胞の分布が変動し，あるマクロファージとT細胞で特異的にオートファジー発現の変動を認めたため，オートファジーがインプラント周囲炎と関連していることが分かった．