2021 Fiscal Year Final Research Report

Identification of pathogenesis of ovarian clear cell carcinoma by topographic single cell sequencing

Research Project

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Project/Area Number	20K21647
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
Research Institution	Niigata University
Principal Investigator	Enomoto Takayuki 新潟大学, 医歯学系, 教授 (90283754)
Co-Investigator(Kenkyū-buntansha)	井ノ上逸朗国立遺伝学研究所, ゲノム・進化研究系, 教授 (00192500) 吉原弘祐新潟大学, 医歯学系, 研究准教授 (40547535) 安達聡介新潟大学, 医歯学総合病院, 助教 (50613147) 田村亮新潟大学, 医歯学総合病院, 助教 (70650620) 石黒竜也新潟大学, 医歯学総合病院, 助教 (80625690)
Project Period (FY)	2020-07-30 – 2022-03-31
Keywords	卵巣癌 / 明細胞癌 / 子宮内膜症 / 子宮内膜 / ゲノム
Outline of Final Research Achievements	We aimed to identify genomic and epigenomic alterations from endometriosis to ovarian cancers. Single-cell sequencing analysis will reveal the genomic and epigenomic abnormalities that accumulate during the transition from endometriosis to ovarian clear cell carcinoma and elucidate the pathogenic mechanism of ovarian clear cell carcinoma. To this end, we sampled normal endometrium, endometriosis, and ovarian clear cell carcinoma, and performed whole-exome sequencing for normal endometrium, endometriosis, atypical endometriosis, and carcinoma. In addition, we isolated single glands from normal endometrium tissue by laser microdissection, and conducted whole genome sequencing for them. We confirmed that we could extract DNA from cells after laser microdissection and perform whole genome sequencing.
Free Research Field	婦人科腫瘍
Academic Significance and Societal Importance of the Research Achievements	卵巣明細胞がんは日本で頻度の高い卵巣がんであり、プラチナ併用化学療法に抵抗性を示すことから、発症予防が重要になる。正常子宮内膜から子宮内膜症、明細胞癌に移行するにつれて蓄積されるゲノム異常を同定することができ、今後子宮内膜症や卵巣明細胞癌の発症予防に役立つ情報を得ることができた。