High throughput screening for G protein-coupled receptor(s) activated in response to estrogen by RNAi collection

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Yamaguchi University
• Principal Investigator: MIZUKAMI Yoichi 山口大学, 総合科学実験センター, 教授 (80274158)
• Co-Investigator(Kenkyū-buntansha): YOKOMIZO Takehiko 九州大学, 医学研究院, 教授 (60302840)
• Project Period (FY): 2009 – 2011
• Keywords: 女性ホルモン / 細胞膜受容体 / G蛋白結合型受容体 / RNAi / スクリーニング

Research Abstract

Estrogen induces the increase of intracellular Ca^<2+> concentrations in addition to transcriptional activity via nuclear receptor, but the membrane receptor except for GPR30 remains unknown. We have produced RNAi collection for GPCR, and examined the Ca^<2+> response and cell impedance in response to estrogen in the presence of RNAi collection. The membrane receptor did not be identified under the experimental conditions due to the weak response of estrogen. The other assay system will be requested to elucidate the estrogen membrane receptor.

Research Products

• [Journal Article] Optimizing high-resolution melting analysis for the detection of mutations of GPR30/ GPER-1 in breast cancer (2012)
  • Author(s): Aihara, M., Yamamoto, S., Nishioka, H., Hamano, K., Oka, M., and Mizukami, Y.
  • Journal Title: Gene Volume: (in press)
  • Peer Reviewed
• [Journal Article] A high-affinity monoclonal antibody against the FLAG tag useful for G-protein coupled receptor study (2012)
  • Author(s): Sasaki, F., Okuno, T., Saeki, K., Min, L., Onohara, N., Kato, H., Shimizu, T. and Yokomizo, T.
  • Journal Title: Anal. Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] 細胞膜エストロゲン受容体GPER/ GPR30は,なぜ小胞体で見つかったのか (2012)
  • Author(s): 水上洋一
  • Journal Title: 日本薬理学会誌
• [Journal Article] International Union of Basic and Clinical Pharmacology. LXXXIV : Leukotriene Receptor Nomenclature, Distribution, and Pathophysiological Functions (2011)
  • Author(s): Back, M., Dahlen, S. E., Drazen, J. M., Evans, J. F., Serhan, C. N., Shimizu, T., Yokomizo, T. and Rovati, G. E.
  • Journal Title: Pharmacol Volume: 63 Pages: 539-584
  • Peer Reviewed
• [Journal Article] Alterations in adhesion junction precede gap junction remodeling during the development of heart failure in cardiomyopathic hamster. (2011)
  • Author(s): Yoshida M, Ohkusa T, Nakashima T, Takanari H, Yano M, Takemura G, Honjo H, Kodama I, Mizukami Y, Matsuzaki. M.
  • Journal Title: Cardiovasc Volume: 92(1) Pages: 95-105
  • Peer Reviewed
• [Journal Article] Operative injury accelerates tumor growth by inducing mobilization and recruitment of bone marrow-derived stem cells. (2011)
  • Author(s): Takemoto Y, Li TS, Kubo M, Ohshima M, Ueda K, Harada E, Enoki T, Okamoto M, Mizukami Y, Murata T, Hamano K.
  • Journal Title: Surgery Volume: 149(6) Pages: 792-800
  • Peer Reviewed
• [Journal Article] Proteomic Analysis of Proteins Expressing in Regions of Rat Brain by Combination of SDS-PAGE with Nano-Liquid Chromatography-Quadrupole Tandem Mass spectrometry (2010)
  • Author(s): Katagiri, T., Hatano, N., Aihara, M., Kawano, H., Okamoto, M., Xing, L., Izumi, T., Maekawa, T., Nakamura, S., Ishihara, T., Shirai, M., Mizukami, Y.
  • Journal Title: Proteome Science Volume: 8(1): 41
  • Peer Reviewed
• [Journal Article] In vivo functions of GPR30/ GPER-1, a membrane receptor for estrogen-From discovery to functions in vivo- (2010)
  • Author(s): Mizukami, Y.
  • Journal Title: Endocrine J. Volume: 57(2) Pages: 101-107
  • Peer Reviewed
• [Journal Article] Neurtrophil elastase inhibitor, ONO-5046, attenuates hippocampal neuronal damage after transient forebrain ischemia in rats (2009)
  • Author(s): Matayoshi, H., Hirata, T., Yamashita S., Ishida, K., Mizukami, Y., Gondo, T., Matsumoto, M., Sakabe, T.
  • Journal Title: Brain Res Volume: 1259 Pages: 98-106
  • Peer Reviewed
• [Journal Article] Repeated preconditioning with hyperbaric oxygen induces neuroprotection against forebrain ischemia via suppression of p38 mitogen activated protein kinase (2009)
  • Author(s): Yamashita, S., Hirata, T., Mizukami, Y. Cui, Y. J., Fukuda, S., Ishida, K., Matsumoto, M., Sakabe, T.
  • Journal Title: Brain Res. Volume: 1301 Pages: 171-179
  • Peer Reviewed
• [Journal Article] 性ステロイドホルモンとGPCR (2009)
  • Author(s): 水上洋一
  • Journal Title: 実験医学 Volume: 8 Pages: 2080-2086
• [Presentation] Generation of a novel anti-FLAG monoclonal antibody (2012)
  • Author(s): Yokomizo T.
  • Organizer: The 7th Korea-Japan Conference on Cellular Signaling for Young Scientists
  • Place of Presentation: Ulsan, Korea
  • Year and Date: 20120217-18
• [Presentation] Crucial role of the leukotriene B4 receptor 2, BLT2 in epidermal wound healing.(Oralaward) (2011)
  • Author(s): Liu M., Saeki K., Yokomizo T.
  • Organizer: The 10th Global COE International Symposium & 7th Young Investigators Forum
  • Place of Presentation: Singapore Singapore
  • Year and Date: 2011-12-23
• [Presentation] Impact of a 3-linked galactose-containing oligosaccharides of a recombinant mouse/ human chimeric IgG mutant on its Fc effector functions (2010)
  • Author(s): Yusuke Mimura, Ronan Kelly, Yoichi Mizukami, Margaret Goodall, Roy Jefferis, Pauline M Rudd
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, Japan Portpia Hall
  • Year and Date: 20100822-27
• [Presentation] Arrest of spermatogenesis at round spermatids in PLCZ1-deficient mice. (2010)
  • Author(s): Masahiko Ito, Kentaro Nagaoka, Keiji Kuroda, Natsuko Kawano, Keiichi, Yoshida, Yuichirou Harada, Tomohide Shikano, Mami Miyado, Shoji Oda, Kiyotaka Toshimori, Youichi Mizukami, Tomoaki Murata, Akihiro Umezawa, Shunichi Miyazaki, Kenji Miyado.
  • Organizer: 11th International Symposium on Spermatology
  • Place of Presentation: Okinawa, Japan conbention center
  • Year and Date: 20100624-29
• [Presentation] 新規細胞膜エストロゲン受容体GPR30遺伝子の未知変異検出法の確立 (2010)
  • Author(s): 相原正宗, 山本滋, 西岡弘子, 岡本まり子, 岡正朗, 水上洋一
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸市神戸市民会館
  • Year and Date: 2010-12-07
• [Presentation] 乳がん組織における細胞膜女性ホルモン受容体GPR30の遺伝子変異の検出 (2010)
  • Author(s): 相原正宗, 山本滋, 西岡弘子, 岡本まり子, 岡正朗, 水上洋一
  • Organizer: 第111回山口大学医学会学術講演会
  • Place of Presentation: 宇部市山口大学霜仁会館
  • Year and Date: 2010-07-18
• [Presentation] 細胞膜女性ホルモン受容体GPR30の高速未知変異遺伝子検出法の確立と疾患への応用 (2010)
  • Author(s): 相原正宗, 山本滋, 西岡弘子, 岡本まり子, 岡正朗, 水上洋一
  • Organizer: 日本生化学会中四国地方会
  • Place of Presentation: 山口市山口大学大学会館
  • Year and Date: 2010-05-15
• [Presentation] TNFαシグナル伝達経路における新規細胞膜受容体GPR30の関与について (2010)
  • Author(s): 岡本まり子, 相原正宗, 水上洋一
  • Organizer: 日本生化学会中四国地方会
  • Place of Presentation: 山口市山口大学大学会館
  • Year and Date: 2010-05-15
• [Book] イラストレイテッドハーパー、生化学原書 (2011)
  • Author(s): 横溝岳彦
  • Publisher: 丸善
• [Remarks]
  • URL: http://gene.yamaguchi-u.ac.jp/
• [Patent(Industrial Property Rights)] エストロゲン疾患の判別方法 (2010)
  • Inventor(s): 水上洋一
  • Industrial Property Rights Holder: 山口大学
  • Patent Publication Number: 特許、特開2012-34633
  • Filing Date: 2010-08-06