2012 Fiscal Year Final Research Report
Development of the auxin inducible degron system for the study of cultured cells and animals
| Project/Area Number |
21681027
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied genomics
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| Research Institution | National Institute of Genetics (2010-2012)
Osaka University (2009) |
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Principal Investigator |
KANEMAKI Masato 国立遺伝学研究所, 新分野創造センター, 准教授 (20444507)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 条件特異的変異株 |
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| Research Abstract |
We have successfully transplanted a plant specific degradation pathway, that is controlled by the plant hormone auxin, to non-plant cells. This new technology, termed the auxin-inducible degron (AID) system, allows us to deplete a protein of interest in an auxin dependent manner. We showed that AID works in budding and fission yeasts, as well as in chicken DT40, mouse and human cells, and the results were published. We also applied the AID technology for the characterization of proteins in budding yeast and the results were published. Moreover, we successfully made an improved version of AID and applied for a patent.
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