Genome mining and microbial production of secondary metabolites

2011 Fiscal Year Final Research Report

• Project/Area Number: 21688009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: University of Shizuoka (2010-2011); The University of Tokyo (2009)
• Principal Investigator: FUNA Nobutaka 静岡県立大学, 食品栄養科学部, 准教授 (70361574)
• Project Period (FY): 2009 – 2011
• Keywords: ポリケタイド / III型ポリケタイド合成酵素

Research Abstract

Genome mining is an innovative approach to isolating novel natural products by utilizing the genome data of bacteria, fungi, and plants. We characterized novel type I and type III polyketide synthases by genome mining studies of Streptomyces, Aspergillus, and Oryza sativa. We manipulated polyketide biosynthesis to generate libraries of "unnatural" natural products by microorganisms.

Research Products

• [Journal Article] The omethyltransferase SrsB catalyzes the decarboxylative methylation of alkylresorcylic acid during phenolic lipid biosynthesis by Streptomyces griseus (2012)
  • Author(s): Nakano, C., Funa, N., Ohnishi, Y., and Horinouchi, S
  • Journal Title: J. Bacteriol Volume: 194 Pages: 1544-1551
  • DOI: DOI:10.1128/JB.06406-11
  • Peer Reviewed
• [Journal Article] Fatty acyl-AMP ligase involvement in the production of alkylresorcylic acid by a Myxococcus xanthus type III polyketide synthase (2011)
  • Author(s): Hayashi, T., Kitamura, Y., Funa, N., Ohnishi, Y. and Horinouchi, S
  • Journal Title: Chembiochem Volume: 12 Pages: 2166-2176
  • DOI: DOI:10.1002/cbic.201100344
  • Peer Reviewed
• [Journal Article] Alkylresorcylic acidsynthesis bytype III polyketide synthase from Oryza sativa (2010)
  • Author(s): Matsuzawa, M., Katsuyama, Y., Funa, N., and Horinouchi, S
  • Journal Title: Phytochemistry Volume: 71 Pages: 1059-1067
  • DOI: DOI:10.1016/j.phytochem.2010.02.012
  • Peer Reviewed
• [Journal Article] Precursordirected biosynthesis of curcumin analogs in Escherichia coll (2010)
  • Author(s): Katsuyama, Y., Hirose, Y., Funa, N., Ohnishi, Y., and Horinouchi, S
  • Journal Title: Biosci. Biotech. Biochem Volume: 74 Pages: 641-645
  • DOI: DOI:10.1271/bbb.90866
  • Peer Reviewed
• [Journal Article] Physically discreteβ-lactamase-type thioesterase catalyzes product release in atrochrysone synthesis by iterative type I polyketide synthase (2009)
  • Author(s): Awakawa, T., Yokota, K., Funa, N., Doi, F., Mort, N., Watanabe, H., and Horinouchi, S
  • Journal Title: Chem. Biol Volume: 16 Pages: 613-623
  • DOI: DOI:10.1016/j.chembiol.2009.04.004
  • Peer Reviewed
• [Journal Article] Biosynthesis of Aliphatic Polyketides by Type III Polyketide Synthase and Methyltransferase in Bacillus subtilis (2009)
  • Author(s): Nakano, C., Ozawa, H., Akanuma, G., Funa, N., and Horinouchi, S
  • Journal Title: J. Bacteriol Volume: 191 Pages: 4916-4923
  • DOI: DOI:10.1128/JB.00407-09
  • Peer Reviewed
• [Journal Article] Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa (2009)
  • Author(s): Katsuyama, Y., Kita, T., Funa, N., and Horinouchi, S
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 11160-11170
  • DOI: DOI:10.1074/jbc.M90007200
  • Peer Reviewed
• [Presentation] Rhodococcus jostii RHAl株におけるβ-ケトアジピン酸経路の新規バイパス経路の解析 (2011)
  • Author(s): 山梨智也、原啓文、鮒信学
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 20110300
• [Presentation] Microbial production of plant polyketides by utilizing diversereactions of type III polyketide synthases (2011)
  • Author(s): Nobutaka Funa
  • Organizer: IUMS(International Congress of Bacteriology and Applied Microbiology)
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2011-09-09
• [Presentation] 「組換え大腸菌と組換え酵母の共培養による非天然型イソフラボンの生産」 (2010)
  • Author(s): 勝山陽平、松井美里、鮒信学、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] 1型ポリケタイド合成酵素から生成物を解離するβ-1actamse型チオエステラーゼの機能解析 (2010)
  • Author(s): 淡川孝義、横田康介、鮒信学、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] Myxococcus xanthus由来のfattyacyl-AMP ligase依存的priming機構を持ったIII型ポリケタイド合成酵素の機能解析 (2010)
  • Author(s): 林貴之、鮒信学、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] Actinoplanes missouriensis由来の新規テルペノイドーポリケタイド融合化合物合成に関わる遺伝子クラスターの機能解析 (2010)
  • Author(s): 淡川孝義、鮒信学、藤田信之、早川正幸、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] 窒素固定細菌由来III型ポリケタイド合成酵素の環化機構の解析 (2010)
  • Author(s): 佐藤龍太朗、宮永顕正、小沢弘樹、鮒信学、宮園健一、田之倉優、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] ウコン由来クルクミン合成酵素(CURS)の結晶構造解析 (2010)
  • Author(s): 勝山陽平、宮園健一、鮒信学、田之倉優、大西康夫、堀之内末治
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] Curcuminoid synthesis by type III polyketide synthases and their application to microbial production of plant polyketides (2010)
  • Author(s): Nobutaka Funa
  • Organizer: Pacifichem 2010
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2010-12-15
• [Presentation] Aβ-lactamase-type thioesterase responsible for product release in polyketide biosynthesis (2010)
  • Author(s): Nobutaka Funa
  • Organizer: 2010 RIKEN Chemical Biology International Symposium
  • Place of Presentation: Saitama, Japan
  • Year and Date: 2010-10-26
• [Presentation] Combinatorial biosynthesis of plant medicinal polyketides in microorganisms (2009)
  • Author(s): Nobutaka Funa
  • Organizer: 25^<th> Naito Conference
  • Place of Presentation: Hokkaido, Jappan
  • Year and Date: 2009-09-11