Functional analysis of exosomes derived from stem cells from human exfoliated deciduous teeth and application to guiding bone regeneration in the jaw cleft

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Tanimoto Kotato 広島大学, 医系科学研究科(歯), 教授 (20322240)
• Co-Investigator(Kenkyū-buntansha): 吉子 裕二 広島大学, 医系科学研究科(歯), 教授 (20263709) ; 加藤 功一 広島大学, 医系科学研究科(歯), 教授 (50283875) ; 國松 亮 広島大学, 医系科学研究科(歯), 准教授 (40580915)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: エクソソーム / 再生医療 / 乳歯歯髄由来幹細胞

Outline of Final Research Achievements

SHED is classified into several types based on surface antigens, among them, CD146-positive cells are suggested to promote angiogenesis in regenerated tissue through interaction with vascular endothelial cells, and are suggested to play an important role in bone regeneration. The particle size of the obtained SHED-derived exosome was 60-90 nm, and it was revealed that adding it to the culture medium enhanced the calcification ability of bone marrow-derived mesenchymal stem cells (BMSCs). It was also suggested that this effect is mediated by the MAPK pathway. Although DPSCs-derived exosome tended to enhance the calcification ability of BMSCs as well as SHED-derived exosome, no significant difference was observed between DPSCs- and SHED- derived exosomes.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

本研究により、乳歯歯髄由来未分化間葉系幹細胞(SHED)から産生されるエクソソームが分離され、骨髄由来未分化間葉幹細胞に対する骨誘導能が明らかとなった。口唇裂・口蓋裂における顎裂部の骨再生医療へのSHED由来エクソソームの臨床応用の可能性が示されたことは、骨移植などの現行治療に比較して低侵襲の治療の達成につながることから、社会的意義があると考えられる。