2023 Fiscal Year Final Research Report

Prevention and Treatment of Age-Related Macular Degeneration by a Novel Functional Monosaccharide with AMPK-regulated Antioxidant Activity

Research Project

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Project/Area Number	21K11626
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 59040:Nutrition science and health science-related
Research Institution	Kagoshima University
Principal Investigator	Terasaki Hiroto 鹿児島大学, 医歯学域鹿児島大学病院, 准教授 (20746888)
Co-Investigator(Kenkyū-buntansha)	高田聖也鹿児島大学, 医歯学総合研究科, 特任助教 (00878283) 坂本泰二鹿児島大学, 医歯学域医学系, 教授 (10235179) 川原幸一大阪工業大学, 工学部, 教授 (10381170) 山下勝鹿児島大学, 医歯学域医学系, 教授 (10635519) 丸山征郎鹿児島大学, 医歯学総合研究科, 客員研究員 (20082282) 大塚章太郎鹿児島大学, 医歯学総合研究科, 特任助教 (80849901)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	網膜色素上皮細胞 / 希少糖
Outline of Final Research Achievements	Experiments were performed on human retinal pigment epithelial cells in a state of cell polarity by adjusting the culture conditions. The antioxidant effect of hydrogen peroxide stimulation on cells co-cultured with rare sugars was evaluated, and although there was a tendency for rare sugars to exhibit antioxidant effects, no statistically significant differences were obtained. We were considering adjusting the experimental conditions, but Lonza's cells were no longer available, so we measured inflammatory cytokines in intraocular fluid obtained from vitrectomies for various diseases to investigate retinal diseases suitable for treatment with rare sugars in the future, and determined that diabetic retinopathy was suitable as a target disease. Molecule X was also found to be elevated in diabetic retinopathy, and a comparison of the effects of aflibercept and ranibizumab administration showed that ranibizumab reduced the concentration of Molecule X more strongly than aflibercept.
Free Research Field	眼科　網膜硝子体分野
Academic Significance and Societal Importance of the Research Achievements	使用予定であった網膜色素上皮細胞が販売中止となってしまうなど、研究が予定通りに進まないこともあったが抗炎症作用が期待されている希少糖がその効果を発揮できる可能性がある網膜疾患を硝子体中の炎症性サイトカイン濃度を測定することで絞ることができた。また、今回測定したの炎症性サイトカインの一つが現在眼科臨床で頻用されている抗VEGF薬であるラニビズマブやアフリベルセプト投与後に異なる反応を呈しており、網膜疾患治療における薬剤の使い分けを基礎医学的な見地から提唱できることが期待される。