2022 Fiscal Year Final Research Report
Role of ubiquitination in antiviral immune response
Project/Area Number |
21K20625
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0701:Biology at molecular to cellular levels, and related fields
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Research Institution | The University of Tokyo |
Principal Investigator |
Kato Kazuki 東京大学, 先端科学技術研究センター, 講師 (10903776)
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Project Period (FY) |
2021-08-30 – 2023-03-31
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Keywords | 自然免疫 / 抗ウイルス応答 / 核酸認識 / 翻訳後修飾 / ユビキチン |
Outline of Final Research Achievements |
cGAS, an innate immune sensor, recognizes viral DNA and orchestrates host immune responses. Through a combination of biochemical assays and structural biology techniques, we aimed to unravel the underlying mechanism of mono-ubiquitin-dependent activation mediated by cGAS. To accomplish this, we employed in vitro techniques to prepare the mono-ubiquitinating enzyme TRIM56 and performed ubiquitination reactions. Notably, our efforts culminated in the successful generation of ubiquitinated cGAS through the in vitro ubiquitination reaction.
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Free Research Field |
自然免疫
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Academic Significance and Societal Importance of the Research Achievements |
cGASはDNAウイルスに対する重要なDNA受容体であるにも関わらず、翻訳後修飾による活性化機構はよくわかっていない。 本研究において調製したモノユビキチン化cGASを用いることで生化学・構造生物学的解析が可能となり、TRIM56によるモノユビキチン化の生理機能が明らかになることが期待される。
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