2012 Fiscal Year Final Research Report
Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.
| Project/Area Number |
22247012
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
SHIMADA Hideo 兵庫県立大学, 大学院・生命理学研究科, 教授 (80095611)
MIYAZAWA Otsuo 兵庫県立大学, 大学院・生命理学研究科, 教授 (60247252)
MURAMOTO Kazumasa 兵庫県立大学, 大学院・生命理学研究科, 准教授 (50305679)
KAMIYA Katsumasa 筑波大学, 大学院・数理物質系, 助教 (60436243)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生体エネルギー変換 / 膜タンパク質 / 金属タンパク質 / X線結晶構造解析 / 振動分光解析 |
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| Research Abstract |
X-ray structure of bovine heart cytochrome oxidase at the hydrogen atom level showed that the proton-pumping pathway (H-pathway) can accept 4 protons, while the time-resolved infrared analysis showed that the open/closed transition of H-pathway is controlled by the O2 reduction site. The mechanism of suppression of reactive oxygen production by NADH-ubiquinone reductase has been proposed vibrational spectroscopically. The 2D crystallization conditions for FoF1ATP synthase has been essentially established. The induction of conformationl changes in the transmembrane region of CcO by cytocrome c was discovered during the 2D crystallization of the complex of the two proteins.
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