2012 Fiscal Year Final Research Report
Molecular analysis of the interaction of different tissues during organogenesis using mouse-chicken chimera
| Project/Area Number |
22247033
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
AGATA Kiyokazu 京都大学, 大学院・理学研究科, 教授 (70167831)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 器官形成 / ニワトリ胚 / ES細胞 / キメラ解析 / トランスクリプトーム |
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| Research Abstract |
To investigate the interaction of stem cells and the surrounding cells during brain development at the molecular level, we produced mouse ES cell/ chicken embryo chimeras by transplantation of the mouse ES cells into the future brain-forming region of chicken embryos. And then we collected mRNAs from the developing brains and tried to sequence these transcripts using next generation sequencers and to categorize them into mouse- and chicken-derived mRNAs by bioinformatic analysis. We spent a lot of time to collect enough amount of the mRNAs from the developing chimera brains. Thus, we are now trying to sequence them using Illumina Miseq.
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