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2012 Fiscal Year Final Research Report

Generating artificial parenchymal and hollow organs fabricated with collagen fibrils,various cells, and cytokines using a bioreactor that we have developed

Research Project

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Project/Area Number 22300167
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKitasato University

Principal Investigator

ADACHI Eijiro  北里大学, 大学院・医療系研究科, 教授 (30110430)

Co-Investigator(Kenkyū-buntansha) AKAIKE Toshihiro  東京工業大学, 学内共同利用施設等, 教授 (30101207)
TAGAWA Youichi  東京工業大学, 学内共同利用施設等, 准教授 (70262079)
NISHIYAMA Toshio  東京農工大学, 農学部, 教授 (60372455)
MATSUSHITA Osamu  北里大学, 医学部, 教授 (00209537)
HATTORI Masakazu  北里大学, 理学部, 教授 (40211479)
Project Period (FY) 2010 – 2012
Keywords細胞・組織工学 / 積層型高密度培養
Research Abstract

We present a novel approach to generate parenchymal organs, such as liver and hollow tissues, blood vessels, based on the use of cultured cells, atelo collagen I and cytokines. Dense connective tissues were generated simultaneously by accumulating collagen fibrils and fibroblasts on a sheet of polylactic acid mesh or artificial blood vessels of synthetic polymers simultaneously using a bioreactor system that we designed by ourselves. For artificial liver tissues, hepatic cells were introduced into the reactor following the perfusion of another medium containing atelo collagen I. In order to make artificial vessels, cultured endothelial cells were circulated the bioreactor. Embedded hepatic cells can retain their specific functions, such as the albumin and the urea production. The two-layered artificial vessels were well-defined i.e. the layer of collagen fibrils and the endothelial layer. These results suggest that this novel approach can produce biological tissues mechanically solid for surgical operation.

  • Research Products

    (23 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (10 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] In vivo organization of vascularized hepatic tissues model with fibroblast-embedded collagen fibrils formation at intraperitoneal sites in partially hepatectomised mice.2013

    • Author(s)
      M. Tamai, T. Akaike, E. Adachi, and Y.Tagawa
    • Journal Title

      Tissue Engineering PartA

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Epidermal structure created by canine hair follicle keratinocytes enriched with bulge cells in a three-dimensional skin equivalent model in vitro: implications for regenerative therapy of canine epidermis2013

    • Author(s)
      T. Kobayashi, K. Enomoto, Y. H. Wang, J. S. Yoon, R. Okamura, K. Ide, M. Ohyama, T. Nishiyama, T. Iwasaki, and K. Nishifuji
    • Journal Title

      Vet Dermatol

      Volume: 24 (1) Pages: 77-83 e19-20

    • DOI

      DOI:10.1111/j.1365-3164.2012.01097.x

    • Peer Reviewed
  • [Journal Article] Fragility of reconstituted type V collagen fibrils with the chain composition of α1(V) α2(V) α3(V) respective of the D-periodic banding pattern2013

    • Author(s)
      K. Mizuno, H. P. Bachinger, Y. Imamura, T. Hayashi, and E. Adachi
    • Journal Title

      Connect Tissue

      Volume: 54 (1) Pages: 41-48

    • DOI

      DOI:10.3109/03008207.2012.734876.

    • Peer Reviewed
  • [Journal Article] Versican/PG-M is essential for ventricular septal formation subsequent to cardiac atrioventricular cushion development2012

    • Author(s)
      S. Hatano, K. Kimata, N. Hiraiwa, M. Kusakabe, Z. Isogai, E. Adachi, T. Shinomura, and H. Watanabe
    • Journal Title

      Glycobiology

      Volume: 22 (9) Pages: 1268-1277

    • DOI

      DOI:10.1093/glycob/cws095

    • Peer Reviewed
  • [Journal Article] Morphological and histological evaluations of 3D-layered blood vessel constructs prepared by hierarchical cell manipulation2012

    • Author(s)
      M. Matsusaki, K. Kadowaki, E. Adachi, T. Sakura, U. Yokoyama, Y. Ishikawa, and M. Akashi
    • Journal Title

      J Biomater Sci Polym Ed

      Volume: 23 (1-4) Pages: 63-79

    • DOI

      DOI:10.1163/092050610X541953

    • Peer Reviewed
  • [Journal Article] Key role of heparan sulfate chains in assembly of anchoring complex at the dermal-epidermal junction2011

    • Author(s)
      S. Iriyama, M. Tsunenaga, S. Amano, and E. Adachi
    • Journal Title

      Exp Dermatol

      Volume: 20 (11) Pages: 953-955

    • DOI

      DOI:10.1111/j.1600-0625.2011.01329.x

    • Peer Reviewed
  • [Journal Article] Characterization of dense artificial connective tissues generated in a newly designed bioreactor2011

    • Author(s)
      H. Iwashiro, S. Hosoya, K. Hirai, T. Mima, S. Ohashi, T. Aihara, S. Ito, S. Ohara, and E. Adachi
    • Journal Title

      Connect Tissue Res

      Volume: 52 (4) Pages: 340-352

    • DOI

      DOI:10.3109/03008207.2010.531801

    • Peer Reviewed
  • [Journal Article] Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum2010

    • Author(s)
      M. Nagaoka, K. Si-Tayeb, T. Akaike, and S. A. Duncan
    • Journal Title

      BMC Dev Biol

      Volume: 10 Pages: 60

    • DOI

      DOI:10.1186/1471-213X-10-60.

    • Peer Reviewed
  • [Presentation] Morphological Characterization of Vascular Intima Artificially Generated in a Newly Designed Bioreactor2013

    • Author(s)
      Mori N and Adachi E.
    • Organizer
      Gordon Research Conference on Collagen
    • Place of Presentation
      New London
    • Year and Date
      20130714-20
  • [Presentation] コラーゲン結合ドメインを持つヒトEGFが三次元培養皮膚モデルの表皮重層化に及ぼす影響2012

    • Author(s)
      笠原薫、松下治、安達栄治郎、西山敏夫 他1名
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      20121214-16
  • [Presentation] Hyperpigmentation in human solar lentigo is promoted by heparanase-induced loss of heparan sulfate chains.2012

    • Author(s)
      Iriyama S., Adachi E. 他5名
    • Organizer
      Asia Society for Pigment Cell Research Congress
    • Place of Presentation
      New Delhi
    • Year and Date
      20121103-04
  • [Presentation] ヒトES/iPS細胞を用いたin vitroヒト肝組織モデルの構築2011

    • Author(s)
      安 成晧、安達栄治郎、 田川陽一他1名
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] Characterization of Artificial Liver Tissues Composed of Primary Hepatocytes Embedded in Dense Collagen Gels2011

    • Author(s)
      Tamai Miho Akaike T., Adachi E., Tagawa Y.
    • Organizer
      Gordon Research Conference on Collagen
    • Place of Presentation
      New London
    • Year and Date
      20110717-22
  • [Presentation] Heparanaseは皮膚モデルにおける表皮の増殖・分化に関与している2011

    • Author(s)
      福島秀和、安達栄治郎 他3名
    • Organizer
      第43回 日本結合組織学会学術大会 第58回 マトリックス研究会大会 合同学術集会
    • Place of Presentation
      大分
    • Year and Date
      20110610-11
  • [Presentation] 三次元培養ヒト皮膚モデル:皮膚の構造と機能の研究ツールとして(皮膚基礎研究クラスターフォーラム2011

    • Author(s)
      西山敏夫
    • Organizer
      第6回教育セミナー
    • Place of Presentation
      東京
    • Year and Date
      2011-07-14
  • [Presentation] 増殖性肝前駆様細胞を用いた高密度コラーゲンゲルによる3次元肝組織化2010

    • Author(s)
      玉井 美保、赤池敏宏、安達栄治郎、田川陽一他2名
    • Organizer
      第32回日本バイオマテリアル学会大会
    • Place of Presentation
      広島
    • Year and Date
      20101129-30
  • [Presentation] 再構成IV型コラーゲン会合体は培養人工皮膚の表皮基底膜細胞増殖を促進する2010

    • Author(s)
      重水 洋平、安達栄治郎、西山敏夫 他5名
    • Organizer
      第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会
    • Place of Presentation
      秋田
    • Year and Date
      20100819-20
  • [Presentation] I型コラーゲン溶液の濃度相転移型液晶化について2010

    • Author(s)
      風間 圭祐、安達栄治郎 他3名
    • Organizer
      第42回日本結合組織学会学術大会・第57回マトリックス研究会大会合同学術集会
    • Place of Presentation
      秋田
    • Year and Date
      20100819-20
  • [Book] 第4章ものつくりに役立つ細胞外マトリックス(コラーゲン、エラスチン、ラミニン、GAG)2011

    • Author(s)
      森奈津希、安達栄治郎
    • Total Pages
      16
    • Publisher
      CMC出版
  • [Book] 再生医療と人工臓器・ドラッグデリバリーの最近の進歩-「治療・診断のための医療材料システムの開発」-中国・日本科学最前線-研究の現場から-2011年版2010

    • Author(s)
      赤池 敏宏 他1名
    • Total Pages
      5
    • Publisher
      科学技術振興機構
  • [Remarks]

    • URL

      http://morpholab.ahs.kitasato-u.ac.jp/adachilabHP/indexaj.html

  • [Patent(Industrial Property Rights)] 高密度培養組織の製造方法及び高密度培養組織2011

    • Inventor(s)
      安達栄治郎,大橋しほ花,平井 和弥
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Industrial Property Number
      特許第4671365号
    • Acquisition Date
      2011-01-28
  • [Patent(Industrial Property Rights)] 粘膜、上皮および鼓膜の少なくとも一つの再生材および再生評価法2010

    • Inventor(s)
      宮下武憲、森望、西望、安達栄治郎、松下治、服部雅一、伊藤周平、坂本恵子
    • Industrial Property Rights Holder
      国立大学法人香川大学、学校法人北里研究所
    • Industrial Property Number
      特願2010-110694号
    • Filing Date
      2010-05-12

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Published: 2014-08-29  

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