Use of long overlapping peptides for cancer vaccine and Treg control for potentiating immune response

2012 Fiscal Year Final Research Report

• Project/Area Number: 22300332
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor immunology
• Research Institution: Kawasaki University of Medical Welfare
• Principal Investigator: NAKAYAMA Eiichi 川崎医療福祉大学, 医療福祉学部, 教授 (60180428)
• Co-Investigator(Kenkyū-buntansha): KAKIMI Kazuhiro 東京大学, 医学部附属病院, 准教授 (80273358)
• Project Period (FY): 2010 – 2012
• Keywords: がん精巣抗原 / 長鎖ペプチド / がんワクチン / 免疫モニタリング / 抗体反応 / CD4, CD8 T 細胞

Research Abstract

We conducted a phase I clinical trials of NY-ESO-1 cancer vaccines using a 20-mer NY-ESO-1f long peptide and 4 different 30-32-mer NY-ESO-1 overlapping peptides with Picibanil OK-432 and Montanide ISA-51. Either vaccine was well tolerated. Immunomonitoring analysis revealed that either vaccine induced antibody, CD4 and CD8T-cell responses in the vaccinated patients. The findings indicated the either long peptide vaccine is promising for immunotherapy.

Research Products

• [Journal Article] NY-ESO-1 antibody as a novel tumor marker of gastric cancer (2013)
  • Author(s): Fujiwara, S., Wada, H., Kawada, J., Kawabata, R., Fujita, J., Hirao, T., Makari, Y., Shibata, K., Iijima, S., Jungbluth, A.A., Nakamura, Y., Takahashi, T., Kurokawa, Y., Yamasaki, M., Miyata, H., Nakajima, K., Takiguchi, S., Nakayama, E., Mori, M., and Doki, Y.
  • Journal Title: Br. J. Cancer Volume: 108(5) Pages: 1119-1125
  • DOI: doi:10.1038/bjc.2013.51. Epub2013 Feb 12
  • Peer Reviewed
• [Journal Article] Ex vivo characterization of γδ T-cell repertoire in patients after adoptive transfer of Vγ9Vδ2 T cells expressing the interleukin-2 receptor β-chain andthe common γ-chain. (2013)
  • Author(s): Izumi T, Kondo M, Takahashi T, Fujieda N, Kondo A, Tamura N, Murakawa T, Nakajima J, Matsushita H, Kakimi K.
  • Journal Title: Cytotherapy Volume: 15(4) Pages: 481-491
  • DOI: doi:10.1016/j.jcyt.2012.12.004
  • Peer Reviewed
• [Journal Article] Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells. (2013)
  • Author(s): Shimizu K, Mizuno T, Shinga J, Asakura M, Kakimi K, Ishii Y, Masuda K, Maeda T, Sugahara H, Sato Y, Matsushita H, Nishida K, Hanada K, Dorrie J, Schaft N, Bickham K, Koike H, Ando T, Nagai R, Fujii S.
  • Journal Title: Cancer Res. Volume: 73(1) Pages: 62-73
  • DOI: doi:10.1158/0008-5472.CAN-12-0759
  • Peer Reviewed
• [Journal Article] Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide. (2013)
  • Author(s): Eikawa, S., Kakimi, K., Idobe, M., Kuzushima, K., Luescher, I., Ohue, Y., Ikeuchi, K., Uenaka, A., Nishikawa, H., Udono, H., Oka, M., and Nakayama, E.
  • Journal Title: Int. J. Cancer Volume: 132(2) Pages: 345-54
  • DOI: doi:10.1002/ijc.27682. Epub 2012 Jul 11
  • Peer Reviewed
• [Journal Article] Serological identification of Tektin5 as a cancer/testis antigen and its immunogenicity (2012)
  • Author(s): Hanafusa T, Mohamed AE, Domae S, Nakayama E, Ono T
  • Journal Title: BMC Cancer Volume: 12 Pages: 520
  • DOI: doi:10.1186/1471-2407-12-520
  • Peer Reviewed
• [Journal Article] Spontaneous antibody, and CD4 and CD8T-cell responses against XAGE-1b(GAGED2a) in non-small cell lung cancer patients. (2012)
  • Author(s): Ohue, Y., Eikawa, S., Okazaki, N., Mizote, Y., Isobe, M., Uenaka, A., Fukuda M., Old, L.J., Oka, M., and Nakayama, E.
  • Journal Title: Int. J. Cancer Volume: 131(5) Pages: E649-658
  • DOI: doi:10.1002/ijc.27359. Epub 2012 Jan 3
  • Peer Reviewed
• [Journal Article] Clinical trial of the intratumoral administration of labeled DC combined with systemic chemotherapy for esophageal cancer. (2012)
  • Author(s): Fujiwara, S., Wada, H., Miyata, H., Kawada, J., Kawabata, R., Nishikawa, H., Gnjatic, S., Sedrak, C., Sato, E., Nakamura, Y., Sakakibara, M., Kanto, T., Shimosegawa, E., Hatazawa, J., Takahashi, T., Kurokawa, Y., Yamasaki, M., Nakajima, K., Takiguchi, S., Nakayama, E., Mori, M.,and Doki, Y.
  • Journal Title: J. Immunotherapy Volume: 35(6) Pages: 513-521
  • DOI: doi:10.1097/CJI.0b013e3182619cb4
  • Peer Reviewed
• [Journal Article] Targeting spatiotemporal expression of CD137 on tumor-infiltrating cytotoxic T lymphocytes as a novel strategy for agonistic antibody therapy. (2012)
  • Author(s): Noji S, Hosoi A, Takeda K, Matsushita H, Morishita Y, Seto Y, Kakimi K.
  • Journal Title: J. Immunother Volume: 35(6) Pages: 460-472
  • DOI: doi:10.1097/CJI.0b013e31826092db
  • Peer Reviewed
• [Journal Article] A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen. (2011)
  • Author(s): Kakimi K, Isobe M, Uenaka A, Wada H, Sato E, Doki Y, Nakajima J, Seto Y, Yamatsuji T, Naomoto Y, Shiraishi K, Takigawa N, Kiura K, Tsuji K, Iwatsuki K, Oka M, Pan L, Hoffman EW, Old LJ, and Nakayama E.
  • Journal Title: International Journal of Cancer Volume: 129(12) Pages: 2836-2846
  • DOI: dio:10.1002/ijc.25955
  • Peer Reviewed
• [Journal Article] Chemokine receptor CXCR3 facilitates CD8(+) T cell differentiation intoshort-lived effector cells leading to memory degeneration. (2011)
  • Author(s): Kurachi M, Kurachi J, Suenaga F, Tsukui T, Abe J, Ueha S, Tomura M, Sugihara K, Takamura S, Kakimi K, Matsushima K.
  • Journal Title: J. Exp. Med Volume: 208(8) Pages: 605-620
  • DOI: doi:10.1084/jem.20102101
  • Peer Reviewed
• [Journal Article] Adoptive immunotherapy for advanced non-small cell lung cancer using zoledronate-expanded γδTcells: a phase I clinical study. (2011)
  • Author(s): Sakamoto M, Nakajima J, Murakawa T, Fukami T, Yoshida Y, Murayama T, Takamoto S, Matsushita H, Kakimi K.
  • Journal Title: J. Immunother Volume: 34(2) Pages: 202-211
  • DOI: doi:10.1097/CJI.0b013e318207ecfb
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies. (2011)
  • Author(s): Hanafusa T, Ali Mohamed Ali ElDib, Kitaoka K, Ohue Y, Nakayama E, and Ono T.
  • Journal Title: Cancer Letter Volume: 301(1) Pages: 57-62
  • DOI: dio:10.1016/j.canlet.2010.10.024.
  • Peer Reviewed
• [Journal Article] Enrichment of Foxp3+ CD4 Tregs in migrated T-cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6. (2010)
  • Author(s): Eikawa, S., Ohue, Y., Kitaoka, K., Aji, T., Uenaka, A., Oka, M., and Nakayama, E.
  • Journal Title: J. Im」munol. Volume: 185(11) Pages: 6734-6740
  • DOI: dio:10.4049/jimmunol.1000225
  • Peer Reviewed
• [Journal Article] Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells fromCHP-NY-ESO-1-vaccinated patients. (2010)
  • Author(s): Mizote, Y., Taniguchi, T., Tanaka, K., Isobe, M., Wada, H., Saika, T., Kita, S., Koide, Y., Uenaka, A., and Nakayama, E.
  • Journal Title: Vaccine Volume: 28(32) Pages: 5338-5346
  • URL: http://www.journals.elsevier.com/vaccine/
  • Peer Reviewed
• [Remarks]
  • URL: http://www.kawasaki-m.ac.jp/resp/