Isolation of the gene conferred astringency in persimmon and its functional analysis for the mechanisms on tannin accumulation.

2013 Fiscal Year Final Research Report

• Project/Area Number: 22380023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kyoto University
• Principal Investigator: YONEMORI Keizo 京都大学, (連合)農学研究科(研究院), 教授 (10111949)
• Co-Investigator(Kenkyū-buntansha): SATO Akihiko 農研機構果樹研究所, ブドウ・カキ研究 領域, 上級研究員 (30355440) ; YAMANE Hisay0 京都大学, 大学院農学研究科, 講師 (80335306)
• Co-Investigator(Renkei-kenkyūsha): KANZAKI Shinya 近畿大学, 農学部, 准教授 (20330243)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Keywords: 園芸学 / プロアントシアニジン / タンニン / ゲノム

Research Abstract

Fosmid library was constructed by a diploid relative, Diospyros lotus, of D. kaki (hexaploid) for isolation of AST gene which confers astringency trait in persimmon. After screening seed clone from fosmid library by using a probe of AST-linked marker, fosmid contig was developed by further screening of the library using end-sequences of the clone. In addition, BAC library of D. lotus was constructed and BAC contig was also developed. Each contig seemed to contain AST locus there, so that we were approaching very near to isolate the AST gene. Furthermore, this BAC library was shown to be effective to isolate the CPCNA gene which confers the trait of non-astringency in Chinese-type non-astringent persimmon. Using the BAC library, isolation of CPCNA gene may also be possible.

Research Products

• [Journal Article] Practical marker-assisted selectiom using two SCAR markers for fruit astringency type in crosses of'Taiten'X PCNA cultivars in persimmon breeding (2013)
  • Author(s): Mitani N., A. Kono, M. Yamada, A. Sato, S. Kobayashi, Y. Ban, T. Ueno, M. Shiraishi, S. Kanzaki, T. Tsujimoto, K. Yonemori
  • Journal Title: Scientica Horticulturae Volume: 170 Pages: 219-223
  • Peer Reviewed
• [Journal Article] Fine genotyping of a highly polymorphic ASTRINGENCY-linked locus reveals variable hexasomic inheritance in persimmon (Diospyros kaki Thunb.) cultivars (2012)
  • Author(s): Akagi T., R. Tao, T. Tsujimoto, A. Kono, K. Yonemori
  • Journal Title: Tree Genomics & Genomes Volume: 8 Pages: 195 -204
  • Peer Reviewed
• [Journal Article] Development of molecular markers linked to the allele associated with the non-astringent trait of the Chinese persimmon (Diospyros kaki Thunb.) (2011)
  • Author(s): Ikegami A., S. Eguchi, T. Akagi, A. Sato, M. Yamada, S. Kanzaki, A. Kitajima, K. Yonemori
  • Journal Title: Journal of the Japanese Society for Horticultural Science Volume: 80 Pages: 150-155
  • Peer Reviewed
• [Journal Article] Proanthocyanidin biosynthesis of persimmon (Diospyros kaki Thunb.) fruits (2011)
  • Author(s): Akagi T., A. Ikegami, K. Yonemori
  • Journal Title: Scientia Horticulturae Volume: 130 Pages: 373-380
  • Peer Reviewed
• [Presentation] カキの甘渋性制御遺伝子(AST)のマッピング (2011)
  • Author(s): 辻本誠幸,赤木剛士,河野淳,三谷宣仁,佐藤明彦,米森敬三
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学農学部
  • Year and Date: 20110000
• [Presentation] カキの甘渋性識別SCARマーカーを利用した完全甘ガキの選抜 (2011)
  • Author(s): 三谷宣仁,河野淳,山田目彦,神崎真哉,佐藤明彦,小林省蔵,伴雄介,上野俊人,白石美樹夫,赤木剛士,辻本誠幸,米森敬三
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮大学農学部
  • Year and Date: 20110000
• [Presentation] Diploid/hexaploid syntenic shuttle mapping for the fruit-specific proanthocyanidin regulating gene (Astringency gene) in persimmon (Diopsyros kaki Thunb.) (2011)
  • Author(s): T. Akagi, T. Tsujimoto, A. Sato, A. Kono, R. Tao, K. Yonemori
  • Organizer: International Plant & Animal Genomes XIX
  • Place of Presentation: Town & Country Convention Center, San Diego, USA
  • Year and Date: 20110000
• [Presentation] Molecular marker for selecting pollination constant and non-astringent (PCNA) type persimmon (2010)
  • Author(s): K. Yonemori, S. Kanzaki, T. Akagi, T. Tsujimoto, A. Sato, M. Yamada
  • Organizer: 28^<th> International Horticultural Congress.
  • Place of Presentation: Losbon Congress Centre, Portuguese
  • Year and Date: 20100000
• [Presentation] カキの遺伝学的解析におけるマメガキの有用性 (2010)
  • Author(s): 辻本誠幸,赤木剛士,神崎真哉,米森敬三
  • Organizer: 園芸学会平成22年度秋季大会
  • Place of Presentation: 大分大学旦野原キャンパス
  • Year and Date: 20100000