2012 Fiscal Year Final Research Report
Long term culture and regulation of differentiation of germ cells from the testis in domestic species
| Project/Area Number |
22380150
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kyoto University |
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Principal Investigator |
IMAI Hiroshi 京都大学, 大学院・農学研究科, 教授 (10303869)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 発生工学 / 生殖幹細胞 |
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| Research Abstract |
A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.
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[Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos2011
Author(s)
iyamoto K, Nagai K, Kitamura N, Nishikawa T, Ikegami H, Binh NT, Tsukamoto S, Matsumoto M, Tsukiyama T, Minami N, Yamada M, Ariga H, Miyake M, Kawarasaki T, Matsumoto K, Imai H
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Journal Title
The Proceedings of the National Academy of Science USA
Volume: 108
Pages: 7040-7045
DOI
Peer Reviewed
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