2012 Fiscal Year Final Research Report
Development of the novel diagnostic method for quantification ofpancreatic beta-cell mass by magnetic resonance imaging
| Project/Area Number |
22390185
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
INAGAKI Nobuya 京都大学, 医学(系)研究科, 教授 (30241954)
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| Co-Investigator(Kenkyū-buntansha) |
TOYODA Kentaro 京都大学, 医学(系)研究科, 講師 (00447971)
MATSUDA Tetsuya 京都大学, 情報学研究科, 教授 (00209561)
TOGASHI Kaori 京都大学, 医学(系)研究科, 教授 (90135484)
木村 寛之 京都大学, 薬学研究科, 助教 (50437240)
上田 真史 京都大学, 医学(系)研究科, 助教 (40381967)
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| Project Period (FY) |
2010 – 2012
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| Keywords | エネルギー / 糖質代謝異常 / イメージング |
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| Research Abstract |
The volume of pancreatic β-cells is known to decrease during development and progression of diabetes. The aim of this study is to develop the technique of measurement of β -cell volume in vivo by using magnetic resonance imaging (MRI). We first evaluated whether pancreatic islets were detected by various analytical parameters of MRI with or without the common enhancerssuch as Gd-solution or not, however, they were not imaged. Second, we tried to perform MRI by using newly developed probe. We synthesized the probe by adding the fluorine to one of the ligands ofglucagon-like peptide-1 receptor (GLP-1R), exendin(9-39), which is specifically expressed on pancreatic β-cells. However, contrary to our preliminary experiment, no signals were detected at all. Same analysis using INS-1 cell line showed similar poor results. The reason of this poor signal was suggested to be due to the extremely low affinity of our fluorine-labeled exendin(9-39) to GLP-1R compared to intact exendins. Therefore, we synthesized new probe that fluorine was added to exendin-4, another GLP-1R ligand, and it showed similar binding affinity to intact exendins. We are now planning to perform MRI analysis by using this new probe.
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[Journal Article] Sasaki, M., Fujimoto, S., Inagaki, N.2013
Author(s)
Sasaki, M., Fujimoto, S., Inagaki, N.
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Journal Title
Diabetes
Volume: 62
Pages: 1996-2003
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[Journal Article] Systems analysis of GLP-1 receptor signaling in pancreatic ・-cells.2011
Author(s)
Takeda, Y., Amano, A., Noma, A., Nakamura, Y., Fujimoto, S., and Inagaki, N
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Journal Title
Am. J. Physiol.-Cell Physiol.
Volume: 301
Pages: C792-803
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[Journal Article] FGF-21 enhances islet engraftment in mouse syngeneic islet transplantation model.
Author(s)
Uonaga, T., Toyoda, K., Okitsu, T., Liu, X.-B., Yamane, S., Uemoto, S., and Inagaki, N.
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Journal Title
Islets.
Volume: 2
Pages: 247-251
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[Presentation] Development of non-invasive PET probe for quantifying pancreatic β-cell mass using fluorine-18-labeled exendin-4. 9th International Diabetes Federation Western Pacific Region Congress2012
Author(s)
Fujimoto, H., Toyoda, K., Kimura, H., Saji, H.,Inagaki, N.
Organizer
4th Scientific Meeting of the Asian Association for the Study of Diabetes.
Place of Presentation
Kyoto, Japan
Year and Date
2012-11-27
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