Activation of regulatory T cells by oral inhabitant bacteria and relationship of their existence and growth of cancer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390352
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Hokkaido University
• Principal Investigator: SHIBATA Ken-ichiro 北海道大学, 大学院・歯学研究科, 教授 (50145265)
• Co-Investigator(Kenkyū-buntansha): HASEBE Akira 北海道大学, 大学院・歯学研究科, 助教 (90281815)
• Project Period (FY): 2010 – 2012
• Keywords: 炎症 / ガン / 制御性T細胞 / 抗腫瘍活性 / 口腔細菌

Research Abstract

Both wild-type (WT) and lipoprotein-deficient (dLP) strains of oral streptococci, S. mutans and S. gordonii, increased the proliferation of CD4+CD25+Foxp3+ regulatory T cells, but reduced their suppressive activity. However, the presence of lipoprotein was not involved in these activities.WT strain of S. gordonii suppressed the growth of B16 melanoma in C57BL/6 mice, whereas dLP strain did not, suggesting that the presence of lipoprotein plays an important role in the anti-tumor activity. In addition, it was found that NK cells, but not cytolytic T cells, were involved in the anti-tumor acitivity.

Research Products

• [Journal Article] Toll-like receptor 2-mediated modulation of growth and functions of regulatory T cells by oral streptococci. (2013)
  • Author(s): A. Saeki, T. Segawa, T. Abe, M. Sugiyama, T. Arimoto, H. Hara, A.Hasebe, M. Ohtani, N. Tanizume, M. Ohuchi, H. Kataoka, M. Kawanami, A. Yokoyama and K. Shibata.
  • Journal Title: Mol Oral Microbiol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy (2013)
  • Author(s): A Hasebe, I Ishikawa, H M Shamsul, M Ohtani, T Segawa, A Saeki, N Tanizume, M Oouchi, Y Okagami, T Okano, and K Shibata
  • Journal Title: Photomed Laser Surg Volume: 31 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Involvement of suppressor of cytokine signaling-1-mediated degradation ofMyD88-adaptor-like protein in the suppression of Toll-like receptor 2-mediated signaling by the murine C-type lectinSIGNR1-mediated signaling (2012)
  • Author(s): M Ohtani , M Iyori , A Saeki, N Tanizume, T Into, A Hasebe , Y Totsuka and K Shibata
  • Journal Title: Cell Microbiol Volume: 14(1) Pages: 40-57
  • Peer Reviewed
• [Journal Article] Regulation of Toll-like receptor signaling by NDP52-mediated selective autophagy is normally inactivated by A20. (2012)
  • Author(s): M Inomata, Niida S., Shibata K, and Into T
  • Journal Title: Cell Mol Life Sci Volume: 69(6) Pages: 963-79
  • Peer Reviewed
• [Journal Article] 口腔マイコプラズマ由来ジアシルリポペプチドFSL-1のin vivoにおける抗腫瘍活性」化学療法の領域 (2012)
  • Author(s): 柴田健一郎
  • Journal Title: 医薬ジャーナル社 Volume: vol.28 Pages: 97-104
  • Peer Reviewed
• [Journal Article] In vivo anti-and pro-tumour activities of the TLR2 ligandFSL-1 (2011)
  • Author(s): K Kiura, A Hasebe, A Saeki, T Segawa, F Okada, H M Shamsul, M Ohtani , T Into, N Inoue, M Wakita, K Shibata
  • Journal Title: Immunobiology Volume: 216 Pages: 891-900
  • Peer Reviewed
• [Presentation] Expression and function of Toll-like receptors and NK activity in oral candidiasis and aging (2013)
  • Author(s): 大内学,佐伯歩、柴田健一郎
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場,千葉
  • Year and Date: 20130318-20
• [Presentation] Recognition of oralstreptococci by intracellular sensorsNODs, and inflammasome (2013)
  • Author(s): 佐伯歩、大谷誠、長谷部晃、中澤太、柴田健一郎
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場,千葉
  • Year and Date: 20130318-20
• [Presentation] 口腔レンサ球菌によるインフラマゾームの活性化 (2013)
  • Author(s): 佐伯歩、柴田健一郎
  • Organizer: 第5回「口腔環境制御研究」カテゴリー集会
  • Place of Presentation: 長崎大学,長崎
  • Year and Date: 2013-02-01
• [Presentation] 口腔連鎖球菌の抗腫瘍活性 (2012)
  • Author(s): 原博志、佐伯歩、長谷部晃、柴田健一郎
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 奥羽大学,郡山
  • Year and Date: 20120914-16
• [Presentation] 微生物由来リポタンパク質・リポペプチドの抗腫瘍活性 (2012)
  • Author(s): 原博志、佐伯歩、長谷部晃、柴田健一郎
  • Organizer: 第79回日本細菌学会北海道支部学術総会
  • Place of Presentation: とかちプラザ,帯広
  • Year and Date: 2012-08-29
• [Presentation] 口腔連鎖球菌のパターン認識受容体による認識とインフラマゾームの活性化 (2012)
  • Author(s): 佐伯歩、杉山正博、瀬川卓、長谷部晃、北川善政、柴田健一郎
  • Organizer: 第79回日本細菌学会北海道支部学術総会.
  • Place of Presentation: とかちプラザ,帯広
  • Year and Date: 2012-08-29
• [Presentation] 制御性T細胞(Treg)の免疫抑制活性に及ぼす口腔連鎖球菌の影響 (2011)
  • Author(s): 佐伯歩、瀬川卓、片岡嗣雄、長谷部晃、有本隆文、五十嵐武、川浪雅光、横山敦郎、柴田健一郎.
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 20110930-1002
• [Presentation] 口腔連鎖球菌の生体からの排除におけるリポタンパク質の影響 (2011)
  • Author(s): 瀬川卓、長谷部晃、佐伯歩、片岡嗣雄、有本隆文、五十嵐武、横山敦郎、川浪雅光、柴田健一郎.
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 長良川国際会議場,岐阜
  • Year and Date: 20110930-1002
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/saikin/achievements.html