2012 Fiscal Year Final Research Report
Experimental determination of haplotypes in radiosensitivity-associated genes
| Project/Area Number |
22510215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
MICHIKAWA Yuichi 独立行政法人放射線医学総合研究所, 緊急被ばく医療研究センター, 主任研究員 (20360688)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ゲノム / 放射線 / 遺伝子 / 核酸 / 1分子計測 |
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| Research Abstract |
In this study, experimental haplotype determination has been conducted using cryopreserved human blood samples. Improvement of technology to remove contaminating small DNA fragments generated by freeze and thaw process was carried out. Four SNPs of the CD44 gene were selected and analysis of 50 frozen human bloods was attempted by this experimental system. Failure of 6 samples indicated that some physical properties of DNA preparation might be affecting suitability for this experimental system. Further improvement should be necessary to overcome this.
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