2012 Fiscal Year Final Research Report
Identification and characterization of SNARE-interacting molecules involved in the tip-growth of root hair of plants.
Project/Area Number |
22570051
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Kyoto Prefectural University |
Principal Investigator |
MASA H Sato 京都府立大学, 大学院・生命環境科学研究科, 准教授 (20283575)
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Co-Investigator(Renkei-kenkyūsha) |
FUKAO Youichiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (80432590)
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Project Period (FY) |
2010 – 2012
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Keywords | 植物分子機能 / 先端成長 |
Research Abstract |
In this study, we investigated the function and subcellular localization of SH3Ps using T-DNA insertion or RNAi knockdown mutants and transgenic plants expressing GFP-fusion proteins. We demonstrated that SH3P1-GFP was localized to various organelles including the plasma membrane, transport vesicles as well as the cell plate of dividing cells. In particular, SH3P1-GFP showed polar localization similar to the localization of PIN2 in epidermal cells, and an actin polymerization inhibitor, latrunculinB, a PI3P kinase inhibitor, wortmannin affected the polarized localization SH3P1. This result indicates that the polar localization of SH3P1-GFP depended on actin cytoskeleton and the endocytotic pathway.
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Research Products
(34 results)
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[Journal Article] VASCULAR PLANT ONE-ZINC FINGER PROTEIN 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsi2013
Author(s)
Yusuke Nakai, Yoichi Nakahira, Hiroki Sumida, Kosuke Takebayashi, Yumiko Nagasawa, Kanako Yamasaki, Masako Akiyama, Masaru Ohme-Takagi, Sumire Fujiwara, Takashi Shiina, Nobutaka Mitsuda, Eiichiro Fukusaki, Yasuyuki Kubo, and Masa H. Sato.
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Journal Title
Plant J
Volume: 73
Pages: 761-775
Peer Reviewed
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[Journal Article] Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thalianaincreases cadmium accumulation and tolerance2012
Author(s)
Masako Kiyono, Yumiko Oka, Yuka Sone, Michitaka Tanaka, Ryosuke Nakamura, Masa H.Sato, Hidemitsu Pan-Hou, Kou Sakabe, Ken-ichiro Inoue.
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Journal Title
Planta
Volume: 235
Pages: 841-850
Peer Reviewed
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[Journal Article] A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA62011
Author(s)
Kazuo Ebine, Masaru Fujimoto, Yusuke Okatani, Tomoaki Nishiyama, Tatsuaki Goh, Emi Ito, Tomoko Dainobu, Aiko Nishitani, Tomohiro Uemura, Masa H. Sato, Hans Thordal-Christensen, Nobuhiro Tsutsumi, Akihiko Nakano, Takashi Ueda.
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Journal Title
Nature Cell Biol.
Volume: 13
Pages: 853-859
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