2012 Fiscal Year Final Research Report
Visualization of peroxisomes and microtubules by GFP marker in a green alga Klebsormidium
| Project/Area Number |
22570060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HASHIMOTO Haruki 東京大学, 大学院・総合文化研究科, 准教授 (90134410)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Makoto 上智大学, 理工学部, 准教授 (90332345)
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| Project Period (FY) |
2010 – 2012
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| Keywords | クレブソルミディウム / 形質転換 / GFP / ペルオキシソーム / 微小管 |
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| Research Abstract |
Exogenous expression of GFP marker genes by particle bombardment methods were employed in order to visualize peroxisomes and microtubules in a green alga Klebsormidium flaccidum. Two GFP-fluoresced cells were observed under the following settings: the Closterium CAB1 or CMV 35S promoters, 0.4 μm particles, 650 psi pressure, and a 12 cm distance for the gold particle bombardment.
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