Role of Wnt signal modulators in malignancy of cancer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22590282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: UCHIDA Kazuhiko 筑波大学, 医学医療系, 准教授 (90211078)
• Co-Investigator(Kenkyū-buntansha): SHIMAZUI Toru 筑波大学, 医学医療系, 教授 (80235613)
• Project Period (FY): 2010 – 2012
• Keywords: Wntシグナル / プロテオミクス / 分子標的薬 / 腎明細胞癌 / 肝細胞がん / 質量分析 / 遺伝子発現解析

Research Abstract

In this study, we analyzed the mechanisms of Wnt signaling modulator in carcinogenesis and malignant transformation. We have found overexpression of novel Wnt modulator. Membrane Protein X (MPX) by ‘omics’ analysis of liver cancer. Functional analysis of MPX by over-expression of cDNA and siRNA knockdown for MPX revealed this new Wnt signaling modulator promote cancer invasion in liver cancer.

Research Products

• [Journal Article] Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. (2013)
  • Author(s): Sugimoto K, Shiraki K, Takei Y, Ito M, Nobori T, Suzuki H, Dissanayaka SK, Meno K, Asashima M, Uchida K
  • Journal Title: Int J Mol Med Volume: Vol. 31 Pages: 943-950
  • DOI: DOI:10.1111/cas.12149
  • Peer Reviewed
• [Journal Article] Systemic transduction of p16INK4A antitumor peptide inhibits the growth of MBT-2 mouse bladder tumor cell line grafts (2013)
  • Author(s): Shimazui T, Yoshikawa K, Miyazaki J, Kojima T, Inai H, Ando S, Uemura H, Uchida K, Nishiyama H
  • Journal Title: Int. J. Oncol Volume: Vo l .42 Pages: 543-54
  • DOI: 10.3892/ijo.2012.1752
  • Peer Reviewed
• [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice (2012)
  • Author(s): Oishi H, Itoh S, Matsumoto K, Ishitobi H, Suzuki R, Ema M, Kojima T, Uchida K, Kato M, Miyata T, Takahashi S
  • Journal Title: J.Biochem Volume: 152 Pages: 549
  • DOI: DOI:10.1093/jb/mvs100
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo. (2012)
  • Author(s): Takase H, Matsumoto K, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
  • Journal Title: Blood Volume: 120 Pages: 914
  • DOI: DOI:10.1182/blood
  • Peer Reviewed
• [Journal Article] 網羅的プロテオミクスによる膵癌のバイオマーカーの探索 (2011)
  • Author(s): 白木克哉, 杉本和史, 内田和彦
  • Journal Title: 胆と膵 Volume: Vol. 32 Pages: 811-815
  • Peer Reviewed
• [Journal Article] c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver (2011)
  • Author(s): Kusakabe M, Hasegawa K, Hamada M, Nakamura M, Ohsumi T, Suzuki H, Tran MT, Kudo T, Uchida K, Ninomiya H, Chiba S, Takahashi S
  • Journal Title: Blood Volume: Vol.118 Pages: 1374-85
  • DOI: DOI:10.1182/blood-2010-08-300400
  • Peer Reviewed
• [Journal Article] FOXO1 and TCF7L2 genes involved in metastasis and poor prognosis in clear cell renal cell carcinoma. Genes Chrom. (2010)
  • Author(s): Kojima T, Shimazui T, Horie R, Hinotsu S, Oikawa T, Kawai K, Suzuki H, Meno K, Akaza H, Uchida K
  • Journal Title: Cancer Volume: Vol.49 Pages: 379-389
  • DOI: DOI:10.1002/gcc.20750
  • Peer Reviewed
• [Journal Article] Up-regulation of glypican-3 in human hepatocellular carcinoma (2010)
  • Author(s): Suzuki M, Sugimoto K, Tanaka J, Tameda M, Inagaki Y, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Ito M, Yoneda M, Uchida K, Takase K, Shiraki K
  • Volume: Vol.30
  • Pages: 5055-61
  • Peer Reviewed
• [Journal Article] Functional cell surface expression of Toll-like receptor 9 promotes cell proliferation and survival in human hepatocellular carcinomas (2010)
  • Author(s): Tanaka J, Sugimoto K, Shiraki K, Tameda M, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Uchida K, Kojima T, Takei Y
  • Journal Title: Int J Oncol Volume: 37 Pages: 805-14
  • Peer Reviewed
• [Journal Article] Laser microdissection-based analysis of cytokine balance in the kidneys of patients with lupus nephritis (2010)
  • Author(s): Wang Y, Ito S, Chino Y, Goto D, Matsumoto I, Murata H, Tsutsumi A, Hayashi T, Uchida K, Usui J, Yamagata K, Sumida T.
  • Journal Title: Clin Exp Immunol. Volume: Vol.159 Pages: 1-10
  • Peer Reviewed
• [Presentation] Peptidomeによるバイオマーカー探索 (2012)
  • Author(s): 内田和彦
  • Organizer: 生命医薬情報学連合大会2012(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-10-14
• [Presentation] Discovery of'cell-derived'circulating peptide biomarker in blood and LC-MS/MS assay developmentfor liver disease. (2012)
  • Author(s): Uchida, K
  • Organizer: AACC2012
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2012-07-15
• [Presentation] dentification of serum biomarkers for transaminase-negative chronic hepatitis and nonalcoholic fatty liver disease by proteomics (2012)
  • Author(s): Uchida K., Suzuki H, Meno K, Korenaga T, Nagashima R, Sugimoto K, Toyota H, Kumada S, Okanoue T, Shiraki K
  • Organizer: The 21th Asian Pacific Association for the Study of the Liver (APASL 2011) (Plenary lecture)
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2012-02-18
• [Presentation] Peptidomic and immunological analyses reveal novel circulating biomarkers for nonalcoholic fatty liver disease (2011)
  • Author(s): Korenaga T, Meno K, Suzuki H, Sugimoto K, Shiraki K, Uchida K
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Pooled Sampleを用いた血清中のNASHバイオマーカーペプチドのiTRAQによる同定 (2011)
  • Author(s): 杉本和史, 山本憲彦, 白木克哉, 竹井謙之, 熊田卓, 内田和彦
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] 二次元電気泳動と多段階質量分析を利用した新規HCV関連肝疾患進展マーカーの検出 (2011)
  • Author(s): 為田雅彦、杉本和史、白木克哉、山本憲彦、竹井謙之、内田和彦
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] 網羅的プロテオミクスによる膵癌のバイオマーカーの探索 (2011)
  • Author(s): 白木克哉、杉本和史、熊田卓、内田和彦
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-15
• [Presentation] New Strategy for Identification and Assay Development of Circulating Peptide Biomarkers for Chronic Liver Disease and Hepatocellular Carcinoma. (2010)
  • Author(s): Uchida K, Suzuki, H., Meno, K., Ishii, T., Sugimoto, K., Nagashima, N., Shiraki, K.
  • Organizer: 国際ヒトプロテオーム機構HUPO 9th World Congres.
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-09
• [Presentation] Quantitative Peptidomic Analysis Reveals Novel Circulating Biomarkers for Chronic Inflammation in Transaminase-Negative Chronic Hepatitis and Nonalcoholic Fatty Liver Disease. (2010)
  • Author(s): Suzuki, H., Meno, K., Korenaga, T., Ishii, T., Sugimoto, K., Nagashima, R., Shiraki, K., Uchida, K
  • Organizer: 国際ヒトプロテオーム機構HUPO 9th World Congres
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-09
• [Presentation] Dishevelled阻害薬(FJ9)における腎癌細胞株のWntシグナルの抑制と抗腫瘍効果に関する検討 (2010)
  • Author(s): 小島崇宏, 島居徹, 及川剛宏, 河合弘二, 内田和彦, 赤座英之
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-04-27