2012 Fiscal Year Final Research Report
Basic research for the comprehensive analysis of selective whitematter injury and the axonal regeneration
| Project/Area Number |
22591577
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IMAI Hideaki 東京大学, 医学部附属病院, 特任講師 (70359587)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Nobuhito 東京大学, 医学部附属病院, 教授 (60262002)
ISHIZAKI Yasuki 群馬大学, 大学院・医学系研究科, 教授 (90183003)
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| Co-Investigator(Renkei-kenkyūsha) |
SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664)
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| Research Collaborator |
堀川 弘吏 東京大学, 大学院生
宮脇 哲 東京大学, 大学院生
小野 秀明 東京大学, 大学院生
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| Project Period (FY) |
2010 – 2012
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| Keywords | 脳梗塞 / 白質障害 / 病態解析 / 質量顕微鏡 / 軸索再生 / 細胞移植治療 |
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| Research Abstract |
Sprague-Dawley rats were used. Under general anesthesia endothelin-1(ET-1:0.5μg/μl) was stereotactically injected into unilateral internal capsule to induce selective white matter damage.Selective white matter injury was evaluated by MRI, and particularly axonal injury was confirmed by specific APP immunoreactivity. The increased immunoreactivitiy for ED1 was observed in the lesion from3 days to 2 weeks after injection showing the activated microglia migrated and occupied the lesion of white matter injury. Imaging mass spectrometry (IMS) revealed the dynamicchange of phosphatidylcholines comprehensively IMS has tremendous potentials to provide the new insight in terms of the dynamic molecular changes invisible under conventional modality.The microvascular endothelial cells (MVECs) were prepared from rat cerebra for the cell transplantation. MVECs were transplanted into the ischemic lesion of internal capsule. MVECtransplantation improved the behavioral outcome and induced remyelion. Also the inflammatory response was repressed by MVEC transplantation. Elucidation of the mechanisms by which MVECs ameliorate ischemic damage of the white matter mayprovide important information for the development of effective therapies for white matter ischemia.
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Author(s)
H Ono, H Nakatomi, K Tsutsumi, T Inoue, A Teraoka, Y Yoshimoto, T Ide, C Kitanaka, K Ueki, H Imai, N Saito.
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Journal Title
Stroke
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Pages: 126-31
DOI
Peer Reviewed
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[Journal Article] ラット選択的白質障害モデルに おけるストレス脆弱性の検討(会議録).2012
Author(s)
小野秀明,今井英明,宮脇哲,堀川弘吏,越智崇,伊藤明博,宮田茂雄,倉知正,石崎泰樹,山形弘隆,三國雅彦,斉藤延人
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脳循環代謝(0915-9401)
Volume: 24巻1号
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Peer Reviewed
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[Journal Article] Cyclophilin C-associated protein regulation of phagocytic functions via NFAT activation in macrophages.2011
Author(s)
Yamaguchi R, Hosaka M, Torii S, Hou N, 英明,中冨 浩文, 小山 博史, 斉藤 延人Saito N, Yoshimoto Y, Imai H, Takeuchi T.
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Journal Title
Brain Res.
Volume: 23
Pages: 55-65
DOI
Peer Reviewed
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[Journal Article] 虚血ストレス下における MKP-1 の発現上昇及び ERK の発現調節を介し たその役割(会議録)脳循環代謝2010
Author(s)
堀川 弘吏, 今井 英明,村田 知里, 小野 秀明, 宮脇 哲, 越智 崇, 伊藤 明博, 鳥居征司, 齊藤 延人
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Journal Title
細胞分化・細胞死 オートファゴ ソーム/リソソーム由来の恒常的 ROS 産生が 酸化ストレス性神経細胞死に関与する(Celldifferentiation and death Implication of constitutive reactive oxygen species generation from autophagosome/lysosome in neuronal oxidative cell death)(英語)(会議録)日本細胞生物学会大会講演要旨集 62回
Peer Reviewed
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