Selective brain tumor cell death caused by synchrotron microbeam X-ray radiation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22591586
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kobe University
• Principal Investigator: KONDOH Takeshi 神戸大学, 大学院・医学研究科, 医学研究員 (50273769)
• Co-Investigator(Kenkyū-buntansha): KOHMURA Eiji 神戸大学, 大学院・医学研究科, 教授 (30225388) ; SASAYAMA Takashi 神戸大学, 医学部附属病院, 講師 (10379399)
• Co-Investigator(Renkei-kenkyūsha): FUKUMOTO Manabu 東北大学, 加齢医学研究所, 教授 (60156809)
• Project Period (FY): 2010 – 2012
• Keywords: 脳腫瘍 / 高輝度放射光 / 放射線治療 / マイクロビーム

Research Abstract

Microbeam radiation therapy (MRT) with wide (100 μm) peak width in co-planar or cross planar manner was applied on human U251 glioma cells and compared with narrow (20 μm) MRT. X-ray was generated at SPring-8, a large scale shynchrotron radiation facility, and newly designed adjustable collimeter was used. Spacing distances on center of beams in wide and narrow width microbeam were 500 μm and 100 μm, setting the same ratio to beam width of five. Narrow width MRT provide sufficient antitumoral effect at the peak dose of 130 Gy. Wide MRT has less effect, but in cross-planar manner or by repeating twice, wide MRT showed comparative effect to narrow MRT. Apoptotic cell death did not increased in neither wide nor narrow MRT, indicating direct cell death did not account for tumor growth suppression. Functional mechanisms in cell and tissue biology is essential for clinical purpose of MRT.

Research Products

• [Journal Article] Supratentorial pure cortical ependymoma (2012)
  • Author(s): Nakamizo S, Sasayama T, Kondoh T
  • Journal Title: J Clin Neurosci Volume: 19(10) Pages: 1453-1455
  • DOI: DOI:10.1016/j.jocn.2011.09.039.
  • Peer Reviewed
• [Journal Article] A narrow microbeam is more effective for tumor growth suppression than a wide microbeam: an in vivo study using implanted human glioma cells (2011)
  • Author(s): Uyama A, Kondoh T, Nariyama N
  • Journal Title: J Synchrotron Radiat Volume: 18 Pages: 671-678
  • DOI: DOI:10.1107/S090904951101185X
  • Peer Reviewed
• [Journal Article] Micro-Scale Dose Distribution of Microplanar X Rays from Synchrotron Radiation: Measurement and Monte Carlo Calculation Progress in NUCLEAR (2011)
  • Author(s): Nariyama N, Umetani K, Shinohara K
  • Journal Title: SCIENCE and TECHNOLOGY Volume: Vol. 2 Pages: 312-317
  • Peer Reviewed
• [Journal Article] Long-term outcome after multidisciplinary approach for leptomeningeal carcinomatosis in a non-small cell lung cancer patient with poor performance status (2011)
  • Author(s): Nagano T, Kotani Y, Kobayashi K
  • Journal Title: Intern Med Volume: 50(24) Pages: 3019-3022
  • Peer Reviewed
• [Journal Article] 空間的分割照射:格子状X線平板ビーム照射 (2010)
  • Author(s): 近藤威、甲村英二、篠原邦夫
  • Journal Title: 新時代の脳腫瘍学 Volume: 68巻 増刊号 10号 Pages: 432-436
• [Presentation] 高輝度放射光を用いたがん治療の研究 (2012)
  • Author(s): 近藤 威、篠原邦夫、 福本 学、成山展照、梅谷啓二
  • Organizer: 第73回応用物理学会学術講演会
  • Place of Presentation: 愛媛
  • Year and Date: 20120909-12
• [Presentation] 白色 X 線マイクロビームの実験動物への照射実験 (2012)
  • Author(s): 近藤 威、福本 学、栗原 愛、梅谷啓二、篠原邦夫
  • Organizer: 日本放射線影響学会第55回大会
  • Place of Presentation: 仙台
  • Year and Date: 20120906-07
• [Presentation] 放射線照射を 1mm 以下に絞ったら何が起きるか? (2012)
  • Author(s): 近藤 威、篠原邦夫、梅谷啓二
  • Organizer: 第3回放射線外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-01-14
• [Presentation] シンポジウム「脳腫瘍の放射線治療に伴う脳壊死-機序と対策」「Microbeam X 線照射による 選択的腫瘍壊死」 (2011)
  • Author(s): 近藤 威
  • Organizer: 日本放射線腫瘍学会第24回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 20111117-19
• [Presentation] Histology of Glioma and Normal Brain Tissue after Microbeam Irradiation (2010)
  • Author(s): Takeshi Kondoh, Atsushi Uyama, Eiji Kohmura, Ai Kurihara, Manabu Fukumoto, Nobuteru Nariyama, Keiji Umetani, and Kunio Shinohara
  • Organizer: 10th International Conference on Biology and Synchrotron Radiation and 6th International Workshop on Medical Applications of Synchrotron Radiation
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20100215-18