functional analysis of transcription factors which control multipotency of mesenchymal stem cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22592068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KAWAMOTO Takeshi 広島大学, 大学院・医歯薬保健学研究院, 助教 (50224861)
• Project Period (FY): 2010 – 2012
• Keywords: 間葉系幹細胞 / 細胞分化 / 転写因子 / 再生医療

Research Abstract

To analyze the function of mesenchymal stem cell-specific transcription factors, we performed overexpression and knockdown experiments of these factors. Knockdown of some transcription factors inhibited growth and osteogenic differentiation of mesenchymal stem cells. SIM2 seems to be the master gene in the cascade of transcriptional regulation in the stem cells. These results indicate that these transcription factors are involved int the growth and differentiation of mesenchymal stem cells.

Research Products

• [Journal Article] Mesenchymal stem cells ameliorate experimental peritoneal fibrosis by suppressing inflammation and inhibiting TGF-beta1 signaling. (2013)
  • Author(s): Ueno, T., Nakashima, A., DOI:, S., Kawamoto, T., Honda, K., Yokoyama, Y., DOI:, T., Higashi, Y., Yorioka, N., Kato, Y., Kohno, N., and Masaki, T
  • Journal Title: Kidney Int Pages: in press
  • DOI: DOI:10.1038/ki.2013.81
  • Peer Reviewed
• [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells (2012)
  • Author(s): Sato, F., Kawamura, H., Wu, Y., Sato, H., Jin, D., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Kato, Y., and Kijima, H
  • Journal Title: Int. J. Mol. Med Volume: 30 Pages: 495-501
  • DOI: DOI:10.3892/ijmm.2012.1037
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver. Biochem (2012)
  • Author(s): Sato, F., Sato, H., Jin, D., Bhawal, U.K., Wu, Y., Noshiro, M., Kawamoto, T., Fujimoto, K., Seino, H., Morohashi, S., Kato, Y., and Kijima, H
  • Journal Title: Biophys. Res. Commun Volume: 419 Pages: 441-446
  • DOI: DOI:10.1016/j.bbrc.2012.02.076
  • Peer Reviewed
• [Journal Article] Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1. (2012)
  • Author(s): Ueshima, T., Kawamoto, T., Honda, K.K., Noshiro, M., Fujimoto, K., Nakao, S., Ichinose, N., Hashimoto, S., Gotoh, O., and Kato, Y.
  • Journal Title: Gene Volume: 510 Pages: 118-125
  • DOI: DOI:10.1016/j.gene.2012.08.022
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells. Biomed Res (2012)
  • Author(s): Wu, Y., Sato, F., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Kato, Y., and Kijima, H
  • Journal Title: Biomed Res. Volume: 33 Pages: 75-82
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22572381.
  • Peer Reviewed
• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu, Y., Sato, F., Yamada, T., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Hakamada, K., Abiko, Y., Kato, Y., and Kijima, H.
  • Journal Title: Int. J. Oncol Volume: 41 Pages: 1337-1346
  • DOI: DOI:10.3892/ijo.2012.1559
• [Journal Article] IL-1beta-mediated up-regulation of DEC1 in human gingiva cells via the Akt pathway (2012)
  • Author(s): Bhawal, U.K., Ito, Y., Tanimoto, K., Sato, F., Fujimoto, K., Kawamoto, T., Sasahira, T., Hamada, N., Kuniyasu, H., Arakawa, H., Kato, Y., and Abiko, Y.
  • Journal Title: J. Cell. Biochem Volume: 113 Pages: 3246-3253
  • DOI: DOI:10.1002/jcb.24205
  • Peer Reviewed
• [Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORalpha and their roles in adipogenesis(dagger) (2012)
  • Author(s): Ozaki, N., Noshiro, M., Kawamoto, T., Nakashima, A., Honda, K., Fukuzaki-Dohi, U., Honma, S., Fujimoto, K., Tanimoto, K., Tanne, K., and Kato, Y
  • Journal Title: Genes Cells Volume: 17 Pages: 109-121
  • DOI: DOI:10.1111/j.1365-2443.2011.01574.x
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D1 (2011)
  • Author(s): Bhawal, U.K., Sato, F., Arakawa, Y., Fujimoto, K., Kawamoto, T., Tanimoto, K., Ito, Y., Sasahira, T., Sakurai, T., Kobayashi, M., Kashima, I., Kijima, H.Kuniyasu, H., Abiko, Y., Kato, Y., and Sato, S.
  • Journal Title: J. Pathol Volume: 224 Pages: 420-429
  • DOI: DOI:10.1002/path.2878
  • Peer Reviewed
• [Journal Article] Impact of zinc fingers and homeoboxes 3 on the regulation of mesenchymal stem cell osteogenic differentiation. (2011)
  • Author(s): Suehiro, F., Nishimura, M., Kawamoto, T., Kanawa, M., Yoshizawa, Y., Murata, H., and Kato, Y
  • Journal Title: Stem Cells Dev Volume: 20 Pages: 1539-1547
  • DOI: DOI:10.1089/scd.2010.0279
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells. (2011)
  • Author(s): Wu, Y., Sato, F., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Morohashi, S., Kato, Y., and Kijima, H.
  • Journal Title: Int. J. Mol. Med. Volume: 27 Pages: 491-495
  • DOI: DOI:10.3892/ijmm.2011.617
  • Peer Reviewed
• [Presentation] 間葉系幹細胞の軟骨分化における DEC2の役割 (2011)
  • Author(s): 笹本智子、藤本勝巳、金輪真佐美、河本健、能城光秀、道田将彦、尾崎徳継、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Presentation] 間葉系幹細胞の増殖・分化ならびにインテクリン発現に対する転写因子 GATA6 の作用 (2011)
  • Author(s): 道田将彦、河本健、能城光秀、藤本勝巳、金輪真佐美、尾崎徳継、笹本智子、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
  • Year and Date: 20110513-14
• [Presentation] Transcription factors regulating differentiation of MSC (2011)
  • Author(s): Kawamoto T., Kubo H., Iwata Y., Fujimoto K., Noshiro M., Kato Y.
  • Organizer: 3rd International Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences
  • Place of Presentation: 広島市
  • Year and Date: 20110128-30
• [Presentation] Identification of HMGA2 and SIM2 interaction in MSC (2011)
  • Author(s): Saskianti T., Kawamoto T., Kato Y.
  • Organizer: 3rd International Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences
  • Place of Presentation: 広島市
  • Year and Date: 20110128-30
• [Presentation] Expression profiles of nuclear receptors and their functions in rat growth plate Chondrocytes (2011)
  • Author(s): Ozaki N., Noshiro M., Honda K., Hayashibara K., Kawamoto T., Fujimoto K., Tanne K., Kato Y
  • Organizer: 3rd International Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences
  • Place of Presentation: 広島市
  • Year and Date: 20110128-30
• [Presentation] Inhibitory Effect of Mesenchymal Stem Cells on the Progression of Experimental Peritoneal Fibrosis in a Rat Model (2010)
  • Author(s): Ueno T., Nakashima A., Taniguchi Y., Kawamoto T., Kato Y., Yorioka N
  • Organizer: 3rd annual meeting of American Society of Nephrology
  • Place of Presentation: Denver, USA.
  • Year and Date: 20101118-21
• [Presentation] 骨髄・滑膜由来間葉系幹細胞におけるインテグリン遺伝子発現への転写因子 GATA6 の作用 (2010)
  • Author(s): 道田 将彦、河本 健、久保 裕嗣、金輪 真佐美、丹根 一夫、加藤 幸夫
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
  • Year and Date: 20100514-15