2011 Fiscal Year Final Research Report
ONE STEP HUMAN iPS CELL INDUCTION AND GENE TARGETING USING VIRAL VECTORS
| Project/Area Number |
22659061
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 相同組換え / iPS細胞 / ウイルスベクター / 再生医療 |
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| Research Abstract |
In order to minimize cell culture for iPSC induction and gene repair, thus reducing the risk of introducing new mutations, we examined a strategy of simultaneous iPSC induction and gene targeting. TIG3 human fibroblasts were subjected to iPSC induction by using the Sendai virus vector, SeVp(KOSM). iPSC-like colonies were grown as a cell populationwithout cloning, and were subsequently transduced with a HDAdV targeting the HPRT locus. The gene targeting frequencies were 3-5% of chromosomal integration, which were equivalent to those with authentic human iPSCs. From the start of iPSC induction to isolation of drug-resistant colonies, it took only 6 weeks.
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