Identification of antigen of IgA and Membranous Nephropathy by On -site proteomics

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659165
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: XU Bo 新潟大学, 医歯学総合研究科, 研究員 (70463982)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737) ; ZHANG Ying 新潟大学, 医歯学総合研究科, 研究員 (00529472)
• Project Period (FY): 2010 – 2012
• Keywords: IgA腎症 / 膜性腎症 / プロテオミクス

Research Abstract

Formation of immune complexes were recognized as key rule involved with IgA nephropathy and membranous nephropathy deeply, but it is still unclear in many cases of antigen of these diseases. In this study, 10 μm thickness sections of kidney biopsy samples were pretreated, and glomeruli were dissected by using laser micro dissection (LMD). Peptides were extracted from collected glomeruli followed by On-Site proteomics method followed LC-MS/MS analysis. Proteins with significant changesbetween the IgA・membranous nephropathy and normal glomerulus were analyzed by bioinformatics tool and pathway analysis of diseases to be explored their pathogenesis of IgA・membranous nephropathy

Research Products

• [Journal Article] Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral dquamous cell carcinoma cells (2013)
  • Author(s): Tsuneki M, MaruyamaS, Yamazaki M, Xu B, Essa Ah, Abe T, Babkair H, Cheng J, Yamamoto T, Saku T
  • Journal Title: BBRC Volume: 434 Pages: 124-130
  • DOI: doi:10.1016/j.bbrc.2013.03.057
  • Peer Reviewed
• [Journal Article] Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry (2013)
  • Author(s): Liu Z, Xu B, Nameta M, Zhang Y, Magdeldin S,Yoshida Y, Yamamoto K, Fujinaka H, Yaoita E, Tasaki M, Nakagawa Y, Saito K, Takahashi K, Yamamoto T
  • Journal Title: Clin Exp Nephrol Volume: 掲載予定
  • DOI: doi:10.1007/s10157-012-0708-1
  • Peer Reviewed
• [Journal Article] Murine colon proteome and characterization of the protein pathways (2012)
  • Author(s): Magdeldin S, Yoshida Y, Li H, Maeda Y, Yokoyama M, Enany S, Zhang Y, Xu B, Fujinaka H, Yaoita E, Sasaki S, Yamamoto T
  • Journal Title: BioData Min Volume: 5 Pages: 11
  • DOI: doi:10.1186/1756-0381-5-11
  • Peer Reviewed
• [Journal Article] Extensive proteomic profiling of the secretome of European community acquired methicillin resistant Staphylococcus aureus clone (2012)
  • Author(s): Enany S, Yoshida Y, Magdeldin S, Zhang Y, Xu B, Yamamoto T
  • Journal Title: Peptides Volume: 37 Pages: 128-37
  • DOI: doi:10.1016/j.peptides.2012.06.011
  • Peer Reviewed
• [Journal Article] Proteomic approach to human kidney glomerulus prepared by laser microdissection from frozen biopsy specimens: exploration of proteome after removal of blood-derived proteins (2012)
  • Author(s): Yoshida Y, Nameta M, Kuwano M, Zhang Y, Xu B, Magdeldin S, Cui Z, Fujinaka H, Yaoita E, Tomonaga T, Yamamoto T
  • Journal Title: Proteomics Clin Appl Volume: 6 Pages: 412-7
  • DOI: doi:10.1002/prca.201200016
  • Peer Reviewed
• [Journal Article] "All and None" Refining Strategy; Fishing Your Correct Protein from Proteomics Ocean (2011)
  • Author(s): Magdeldin S, Yoshida Y, Zhang Y, Xu B, Yaoita E, Yamamoto T
  • Journal Title: J Proteomics Bioinform Volume: 4 Pages: 123-124
  • Peer Reviewed
• [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database (2011)
  • Author(s): Zhang Y, Yoshida Y, Xu B, Magdeldin S, Fujinaka H, Liu Z, Miyamoto M, Yaoita E, Yamamoto T
  • Journal Title: Proteome Sci Volume: 9 Pages: 47
  • DOI: doi:10.1186/1477-5956-9-47
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis (2011)
  • Author(s): Xu B, Zhang Y, Zhao Z, Yoshida Y, Magdeldin S, Fujinaka H, Ismail TA, Yaoita E, Yamamoto T
  • Journal Title: J Proteomics Volume: 74 Pages: 1022-9
  • DOI: doi:10.1016/j.jprot.2011.03.001
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343 Pages: 637-648
  • DOI: doi:10.1007/s00441-010-1117-y
  • Peer Reviewed
• [Presentation] Proteomics analysis of laser microdissected FFPE glomerular sections from human IgA nephropathy biopsy specimens (2012)
  • Author(s): Xu B, Shimada S, Zhang Y, Yoshida Y, Liu Z, Magdeldin S, Fujinaka H, Yaoita E, Yamamoto T
  • Organizer: 第11回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ボス トン(アメリカ合衆国)
  • Year and Date: 20120909-13
• [Presentation] Comparison of on-site proteomics study on laser microdissected FFPE and frozen human glomerulus tissues (2011)
  • Author(s): Xu B, Shimada S, Zhang Y, Magdeldin S, Fujinaka H, Yoshida Y, Yaoita Y, Yamamoto T
  • Organizer: 第10回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ジュネーブ(スイス連邦)
  • Year and Date: 20110904-07
• [Presentation] Proteomics results of small FFPE rat cortex tissues treated by 3 methods (2011)
  • Author(s): Xu B, Zhang Y, Shimada S, Maddeldin S, Yoshida Y, Yaoita E, Yamamot T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
  • Year and Date: 20110728-30
• [Presentation] Proteome research of murine liver tissues of NASH model mouse (2011)
  • Author(s): Xu B, Shimada S, Takamura M, Zhang Y, Azlina E, Magdeldin S, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
  • Year and Date: 20110728-30
• [Presentation] Preparation of sample for proteomics research (2011)
  • Author(s): Xu B, Yoshida Y, Yamamoto T
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
  • Year and Date: 20110728-30
• [Presentation] Proteomics analysis on formalin-fixedparaffin-embedded human glomeruli using laser microdissection and on-site direct trypsin digestion (2011)
  • Author(s): Xu B, Shimada S, Zhang Y, Yoshida Y, Yaoita E, Morita T , Yamamoto T
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20110615-17
• [Presentation] On-site proteomics study on laser microdissected rat renal cortex and human glomerulus tissues (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Magdeldin S, Fujinaka H, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 9th Annual World Congress of Human Proteome Organization (HUPO)
  • Place of Presentation: シドニー(オーストラリア連邦)
  • Year and Date: 20100919-23
• [Presentation] Proteomics analysis on formalin-fixed paraffin-embedded tissue using laser microdissection and on-site direct trypsin digestion (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Ikashima M, Yoshida Y, Yaoita E, Morita T , Yamamoto T
  • Organizer: 第8回日本ヒトプロテオーム機構
  • Place of Presentation: 東京
  • Year and Date: 20100726-28
• [Presentation] Proteomics study of formalin fixed paraffin embedded rat kidney by laser microdissection (2010)
  • Author(s): Xu B, Shimada S, Zhang Y, Ikashima M, Yoshida Y, Yaoita E, Yamamoto T
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-18
• [Remarks]
  • URL: http://hkupp.kir.jp