2013 Fiscal Year Final Research Report
The electrochemical detection method of the carbohydrate-protein interaction for carbohydrate microarray
| Project/Area Number |
22780107
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
KOMBA Shiro 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所食品バイオテクノロジー研究領域, 主任研究員 (20332273)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 分子認識 / 糖鎖 |
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| Research Abstract |
I have synthesized various sugar linkers and these were examined for recognition of lectins by the surface plasmon resonance method and an electrical signal. As a results of the various examinations, the sugar linkers that consist of 11-mercaptoundecanoic acid, 4,7,10-trioxa-1,13-tridecandiamine, and lactose or maltose for a terminal beta-galactose or alpha-glucose respectively were the best structure for electrical measurement (SWV measurement). I have established the technique that detect interaction between sugar and its recognizing protein (lectin etc.) by electrical measurement.
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