2013 Fiscal Year Final Research Report
Research on the identification of growth-stimulating peptides from the cerebral ganglion of Pacific abalone
| Project/Area Number |
23380114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUMURA Seiichi 北里大学, 海洋生命科学部, 准教授 (60224169)
AMANO Haruna 北里大学, 海洋生命科学部, 講師 (50431341)
UCHIDA Katsuhisa 宮崎大学, 農学部, 准教授 (50360508)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | エゾアワビ / 脳神経節cDNAライブラリー / 食欲調節ペプチド / 再生促進ペプチド / 性成熟促進ペプチド / 代謝調節ペプチド |
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| Research Abstract |
Pacific abalone is one of the most important aquaculture species in the Tohoku and Hokkaido areas. The most crucial problem for abalone culture is the characteristically slow growth. In an attempt to understand growth regulation in the abalone, we tried to determined growth-stimulating peptide.
cDNA libraries of the cerebral ganglions of abalone were prepared using total RNAs, and about 3300 colons were used for the sequence analysis. At least 42 peptide homologs were detected, and large number of clone is Neuropeptide Y (NPY) homolog. Two NPY cDNAs and peptides, named abNPY-1 and 2, were determined by cDNA cloning and LC-MS/MS.Higher AbNPY mRNA levels were observed in the larger-sized abalone than in those of smaller abalone. Injection of abNPY to juvenile abalone resulted in an increase in body mass. These results suggest that NPY homolog is present in the cerebral ganglion and this peptide is involved in the regulation of somatic growth of the Pacific abalone.
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Research Products
(34 results)
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[Journal Article] Involvement of insulin-like peptide in long-term synaptic plasticity and long-term memory of the pond snail Lymnaea stagnalis2013
Author(s)
Murakami J, Okada R, Sadamoto H, Kobayashi S, Mita K, Sakamoto Y, Yamagishi M, Hatakeyama D, Otsuka E, Okuta A, Sunada H, Takigami S, Sakakibara M, Fujito Y, Awaji M, Moriyama S, Lukowiak K, Ito E
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Journal Title
J Neurosc
Volume: 33
Pages: 371-381
DOI
Peer Reviewed
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[Journal Article] Effects of growth hormone on the salmon pituitary proteome2012
Author(s)
Kurata Y, Kimura Y, Yamanaka Y, Ishikawa A, Okamoto H, Masaoka T, Nagoya H, Araki K, Moriyama S, Hirano H, Mori T
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Journal Title
J Proteomics
Volume: 75
Pages: 1718-1731
DOI
Peer Reviewed
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[Journal Article] Physiological and molecular endocrine changes in maturing wild sockeye salmon, Oncorhynchus nerka, during ocean and river migration2012
Author(s)
Flores AM, Shrimpton JM, Patterson DA, Hills JA, Cooke SJ, Yada T, Moriyama S, Hinch SG, Farrell AP
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Journal Title
J Comp Physiol B
Volume: 182
Pages: 77-90
DOI
Peer Reviewed
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[Presentation] アワビのニューロペプチドYの同定2014
Author(s)
森山俊介, 川村眞人, 舩山翔平, 天野春菜, 川島祐介, 小寺義男, 内田勝久
Organizer
平成26年度日本水産学会春季大会
Place of Presentation
北海道大学水産学部, 函館市, 北海道
Year and Date
20140327-31
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