2013 Fiscal Year Final Research Report
Molecular mechanism of the selective AID targeting at the IgV-region
| Project/Area Number |
23390122
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 免疫学 / ゲノム / 遺伝子 |
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| Research Abstract |
To elucidate the molecular mechanism of GANP interacted with AID in generation of antibody affinity maturation in B cells, GANP interacting proteins was identified in the nuclear extract from human B cell line Ramos cells. GANP interacts with histones and induces chromatin modification with the acetylation though the GANP HAT domain. GANP binds to the rearranged IgV region. This position is relaxed during IgV transcription. Transcription complex similarly retains during the IgV transcription, which presumably maintains the DNA configuration for continuation and providing the opportunity of AID accession. GANP recruits AID towards the selective site of the rearranged IgV region to augment mutation frequency. We further clarified the role of GANP in the choice of DNA repair pathways after the DNA injury caused by AID at the IgV region gene. GANP is essentially involved in maintenance of DNA and the genome integrity for efficient DNA mutations during antibody affinity maturation.
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Research Products
(34 results)
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[Journal Article] Pcid2 inactivates developmental genes in human and mouse embryonic stem cells to sustain their pluripotency by modulation of EID1 stability2014
Author(s)
Ye B, Dai Z, Liu B, Wang R, Li C, Huang G, Wang S, Xia P, Yang X, Kuwahara K, Sakaguchi N, Fan Z
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Journal Title
Stem Cells
Volume: 32
Pages: 623-635
DOI
Peer Reviewed
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[Journal Article] Infection of adult thymus with murine retrovirus induces virus-specific central tolerance that prevents functional memory CD8+ T cell differentiation2014
Author(s)
Takamura S, Kajiwara E, Tsuji-Kawahara S, Masumoto T, Fujisawa M, Kato M, Chikaishi T, Kawasaki Y, Kinoshita S, Itoi M, Sakaguchi N, Miyazawa M
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Journal Title
PLoS Pathog
Volume: 10
Pages: e1003937
DOI
Peer Reviewed
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[Journal Article] Histopathological evaluation of the diversity of cells susceptible to H5N1 virulent avian influenza virus2013
Author(s)
Ogiwara H, Yasui F, Munekata K, Takagi-Kamiya A, Munakata T, Nomura N, Shibasaki F, Kuwahara K, Sakaguchi N, Sakoda Y, Kida H, Kohara M
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Journal Title
Am. J. Pathol
Volume: 184
Pages: 171-183
DOI
Peer Reviewed
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[Journal Article] CA-S27 : a novel Lewis a associated carbohydrate epitope is diagnostic and prognostic for cholangiocarcinoma2013
Author(s)
Silsirivanit A, Araki N, Wongkham C, Vaeteewoottacharn K, Pairojkul C, Kuwahara K, Narimatsu Y, Sawaki H, Narimatsu H, Okada S, Sakaguchi N, Wongkham S
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Journal Title
Cancer Sci
Volume: 104
Pages: 1278-1284
DOI
Peer Reviewed
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[Journal Article] Broad-spectrum detection of h5 subtype influenza a viruses with a new fluorescent immunochromatography system2013
Author(s)
Sakurai A, Takayama K, Nomura N, Munakata T, Yamamoto N, Tamura T, Yamada J, Hashimoto M, Kuwahara K, Sakoda Y, Suda Y, Kobayashi Y, Sakaguchi N, Kida H, Kohara M, Shibasaki F
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Journal Title
PLoS One
Volume: 8
Pages: e76753
DOI
Peer Reviewed
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[Journal Article] Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival2013
Author(s)
Rezano A, Kuwahara K, Yamamoto-Ibusuki M, Kitabatake M, Moolthiya P, Phimsen S, Suda T, Tone S, Yamamoto Y, Iwase H, Sakaguchi N
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Journal Title
BMC Cancer
Volume: 13
Pages: 562
DOI
Peer Reviewed
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[Journal Article] Selective cell death of p53-insufficient cancer cells is induced by knockdown of the mRNA export molecule GANP2011
Author(s)
Phimsen S, Kuwahara K, Nakaya T, Ohta K, Suda T, Rezano A, Kitabatake M, Vaeteewoottacharn K, Okada S, Tone S, Sakaguchi N
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Journal Title
Apoptosis
Volume: 17
Pages: 679-690
DOI
Peer Reviewed
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