2013 Fiscal Year Final Research Report
Elucidation of transcriptional and epigenomic regulation in the development of heart failure
| Project/Area Number |
23390210
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
KUWAHARA Koichiro 京都大学, 医学(系)研究科(研究院), 講師 (30402887)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKIMI Toshio 京都大学, 大学院・医学研究科, 准教授 (80291946)
NAKAGAWA Yasuaki 京都大学, 大学院・医学研究科, 助教 (70452357)
KINOSHITA Hideyuki 京都大学, 大学院・医学研究科, 助教 (30467477)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKANO Makoto 自治医科大学, 医学部, 教授 (30236252)
ARAI Yuji 独立行政法人国立循環器病研究センター, 分子生物学部, 室長 (30202724)
MORI Yasuo 京都大学, 工学研究科, 教授 (80212265)
NISHIDA Motohiro 九州大学, 薬学研究科, 准教授 (90342641)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 転写調節 / 心不全 |
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| Research Abstract |
We examined the transcriptional and epigenomic regulation of cardiac gene expression during the development of heart failure. We found that NRSF-HDAC complex maintains cardiac electrostability by regulating HCN2 and 4 ion channels expression. In addition, we revealed the important role played by MRTF-A, a rho-dependent co-activator of SRF, in pathological cardiac remodeling. Furthermore, we analyzed the therapeutic effect of TRPC3/6 inhibition on pathological cardiac remodeling.
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[Journal Article] The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis2014
Author(s)
Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K
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Journal Title
PLoS One
Volume: 9(3)
Pages: e92314
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[Journal Article] Identification of Chemicals Inducing Cardiomyocyte Proliferation in Developmental Stage-Specific Manner with Pluripotent Stem Cells2013
Author(s)
Uosaki H, Magadum A, Seo K, Fukushima H, Takeuchi A, Nakagawa Y, Moyes KW, Narazaki G, Kuwahara K, Laflamme M, Matsuoka S, Nakatsuji N, Nakao K, Kwon C, Kass DA, Engel FB, Yamashita JK
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Journal Title
Circ Cardiovasc Genet
Volume: 6(6)
Pages: 624-33
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[Journal Article] Angiotensin II Type 1a receptor signaling directly contributes to the increased arrhythmogenicity in cardiac hypertrophy2013
Author(s)
Yasuno S, Kuwahara K, Kinoshita H, Yamada C, Nakagawa Y, Usami S, Kuwabara Y, Ueshima K, Harada M, Nishikimi T, Nakao K
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Journal Title
Br J Pharmacol
Volume: 170(7)
Pages: 1384-95
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[Journal Article] Increased Expression of HCN Channels in the Ventricular Myocardium Contributes to Enhanced Arrhythmicity in Mouse Failing Hearts2013
Author(s)
Kuwabara Y, Kuwahara K, Takano M, Kinoshita H, Arai Y, Yasuno S, Nakagawa Y, Igata S, Usami S, Minami T, Yamada Y, Nakao K, Yamada C, Shibata J, Nishikimi T, Ueshima K, Nakao K
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Journal Title
J Am Heart Assoc
Volume: 2(3)
Pages: e000150
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[Journal Article] Cardiac nuclear High Mobility Group Box 1 prevents the development of cardiac hypertrophy and heart failure2013
Author(s)
Funayama A, Shishido T, Netsu S, Narumi T, Kadowaki S, Takahashi H, Miyamoto T, Watanabe T, Woo CH, Abe JI, Kuwahara K, Nakao K, Takeishi Y, Kubota I
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Journal Title
Cardiovasc Res
Volume: 99(4)
Pages: 657-64
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[Journal Article] Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure2013
Author(s)
Nishikimi T, Okamoto H, Nakamura M, Ogawa N, Horii K, Nagata K, Nakagawa Y, Kinoshita H, Yamada C, Nakao K, Minami T, Kuwabara Y, Kuwahara K, Masuda I, Kangawa K, Minamino N, Nakao K
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Journal Title
PLoS One
Volume: 8(1)
Pages: e53233
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[Journal Article] Determination of the B-type Natriuretic Peptide Level as a Criterion for Abnormalities in Japanese Individuals in Routine Clinical Practice : The J-ABS Multi-center Study (Japan Abnormal BNP Standard)2013
Author(s)
Kawai M, Yoshimura M, Harada M, Mizuno Y, Hiramitsu S, Shimizu M, Shoda T, Kuwahara K, Miyagishima K, Ueshima K, Nakao K
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Journal Title
Intern Med
Volume: 52(2)
Pages: 171-7
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[Journal Article] Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodeling in mice2012
Author(s)
Minami T, Kuwahara K, Nakagawa Y, Takaoka M, Kinoshita H, Nakao K, Kuwabara Y, Yamada Y, Yamada C, Shibata J, Usami S, Yasuno S, Nishikimi T, Ueshima K, Sata M, Nakano H, Seno T, Kawahito Y, Sobue K, Kimura A, Nagai R and Nakao K
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Journal Title
EMBO J
Volume: 31(23)
Pages: 4428-40
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[Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-a2011
Author(s)
Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, Yamashita JK
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Journal Title
PLoS One
Volume: 6(2)
Pages: e16734
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[Journal Article] Zinc-finger protein 90 negatively regulates neuron-restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes2011
Author(s)
Hata L, Murakami M, Kuwahara K, Nakagawa Y, Kinoshita H, Usami S, Yasuno S, Fujiwara M, Kuwabara Y, Minami T, Yamada Y, Yamada Y, Nakao K, Ueshima K, Nishikimi T, and Nakao K
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Journal Title
J Mol Cell Cardiol
Volume: 50(6)
Pages: 972-981
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[Presentation] Elucidation of molecular mechanism of human proBNP : role of O-glycosylation in N-terminal region of proBNP2014
Author(s)
Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Yamada C, Ueshima K, Minamino N, Kimura T, Nakao K
Organizer
第78回日本循環器学会学術集会
Place of Presentation
東京
Year and Date
20140321-23
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[Presentation] Molecular mechanism of lower processing rate in human proBNP : importance of the distance between the O-glycosylation and cleavage sites2014
Author(s)
Nishikimi T, Nakagawa Y, Minamino N, Ikeda M, Ishimitsu T, Yamada C, Nakao K, Minami T, Kuwabara Y, Kinoshita H, Kangawa K, Kuwahara K, Nakao K
Organizer
第78回日本循環器学会学術集会
Place of Presentation
東京
Year and Date
20140321-23
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[Presentation] O-glycosylated Pro B-type Natriuretic Peptide is Cleaved intracellularly By Furin in Ventricular Myocytes and Atrial Myocytes : Importance of the Distance Between the O-glycosylation Sites and Cleaved Sites2013
Author(s)
Nishikimi T, Nakagawa Y, Minamino N, Ikeda M, Ishimitsu T, Yamada C, Nakao K, Minami T, Kuwabara Y, Kinoshita H, Kangawa K, Kuwahara K, Nakao K
Organizer
AHA Scientific Sessoins 2013
Place of Presentation
Dallas, USA
Year and Date
20131116-20
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[Presentation] Blockade of TRPC6 is a Novel Therapeutic Approach Against Pathological Cardiac Remodeling2013
Author(s)
Kinoshita H, Kuwahara K, Nishida M, Kurose H, Kiyonaka S, Mori Y, Yamada C, Kuwabara Y, Yasuno S, Nakagawa Y, Nishikimi T, Ueshima K, Nakao K
Organizer
International Conference on Endothelin ET-13
Place of Presentation
Tokyo
Year and Date
20130908-11
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[Presentation] Effect of hemodyalysis on precursor proBNP and glycosylated NT-proBNP in end-stage renal disease patients2013
Author(s)
Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Yamada C, Nakao K, Ueshima K, Nakao K
Organizer
European Society of Cardiology 2013 (ESC 2013)
Place of Presentation
Amsterdam, Netherland
Year and Date
20130831-0904
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[Presentation] Glycosylation of plasma N-terminal proBNP and its effect on NT-proBNP assay in hemodyalysis patients2013
Author(s)
Yasuaki Nakagawa, Toshio Nishikimi, , Koichiro Kuwahara Yoshihiro Kuwabara, , Hideyuki Kinoshita, Makoto Takano, Satoru Usami, Kazuhiro Nakao, Takeya Minami, Yuko Yamada, Chinatsu Yamada, Junko Shibata, Kenji Ueshima and Kazuwa Nakao
Organizer
第77回日本循環器学会学術総会
Place of Presentation
横浜
Year and Date
20130315-17
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[Presentation] TRPC3/6 Ion Channels as Potentially Novel Therapeutic Targets against Pulmonary Arterial Hypertension2012
Author(s)
Hideyuki Kinoshita, Koichiro Kuwahara, Shigeki Kiyonaka, Yasuo Mori, Junko Shibata, Chinatu Yamada, Takeya Minami, Kazuhiro Nakao, Yoshihiro Kuwabara, Shinji Yasuno, Satoru Usami, Yasuaki Nakagawa, Toshio Nishikimi, Kenji Ueshima, KazuwaNakao
Organizer
The76th Annual Scientific Meeting of The Japanese CirculationSociety
Place of Presentation
Fukuoka International Congress Center, Fukuoka, Japan
Year and Date
20120316-18
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[Presentation] Blockade of TRPC6/3 is a Novel Therapeutic Approach for Preventing Pathological Cardiac Hypertrophy2011
Author(s)
Hideyuki Kinoshita, Koichiro Kuwahara, Motohiro Nishida, Hitoshi Kurose, Shigeki Kiyonaka, Yasuo Mori, Yoshihiro Kuwabara, Yasuaki Nakagawa, Kenji Ueshima, Kazuwa Nakao
Organizer
European Society of Cardiology Congress
Place of Presentation
Paris, France
Year and Date
20110827-31
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