Role of basement membranes in small-vessel disease of brain

2013 Fiscal Year Final Research Report

• Project/Area Number: 23390348
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Okayama University
• Principal Investigator: NINOMIYA YOSHIFUMI 岡山大学, 医歯(薬)学総合研究科, 教授 (70126241)
• Co-Investigator(Kenkyū-buntansha): YONEZAWA Tomoko 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30304299) ; OOHASHI Toshitaka 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (50194262)
• Co-Investigator(Renkei-kenkyūsha): HIROHATA Satoshi 岡山大学, 国際センター, 准教授 (90332791) ; OHTSUKA Aiji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50168986)
• Research Collaborator: HATANAKA Kunihiko 岡山大学, 医学部, 客員研究員 ; SAITO Kenji 岡山大学, 医学部, 客員研究員 ; MOMOTA Ryusuke 岡山大学, 大学院・医歯薬学総合研究科, 助教 ; OGAWA Hiroko 岡山大学, 大学病院, 助教 ; INAGAKI Jyunko 岡山大学, 大学院・医歯薬学総合研究科, 助教
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 脳小血管障害 / コラーゲン / 血液-脳関門

Research Abstract

Abnormalities of the basement membranes in brain small vessels are considered to cause the break-down of blood-brain barrier (BBB) and intracerebral hemorrhage. We established the mouse encephalopathy model by the administration of TNF-alpha intravenously, in which BBB permeability increase transiently. To know the change of type IV collagen, as major component of the basement membranes, in the encephalopathy model, we performed Western blot and immunohistochemistry. The results suggested that the degradation of type IV collagen was associated with the break-down of BBB.

Research Products

• [Journal Article] ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor (2014)
  • Author(s): Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek MZ, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
  • Journal Title: Exp Cell Res Volume: 323(2) Pages: 263-75
  • DOI: 10.1016/j.yexcr.2014.03.002
  • Peer Reviewed
• [Journal Article] Light and electron microscopic detection of inflammation-targeting liposomes encapsulating high-density colloidal gold in arthritic mice (2014)
  • Author(s): Maehara A, Nishida K, Furutani M, Matsumoto E, Ohtsuka A, Ninomiya Y, Oohashi T
  • Journal Title: Inflamm Res Volume: 63(2) Pages: 139-47
  • DOI: 10.1007/s00011-013-0682-4
  • Peer Reviewed
• [Journal Article] ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse (2013)
  • Author(s): Demircan K, Yonezawa T, Takigawa T, Topcu V, Erdogan S, Ucar F, Armutcu F, Yigitoglu MR, Ninomiya Y, Hirohata S
  • Journal Title: Neurosci Lett Volume: 544 Pages: 25-30
  • DOI: 10.1016/j.neulet.2013.02.064
  • Peer Reviewed
• [Journal Article] Drosophila type XV/XVIII collagen mutants manifest integrin mediated mitochondrial dysfunction, which is improved by cyclosporin A and losartan (2013)
  • Author(s): Momota R, Narasaki M, Komiyama T, Naito I, Ninomiya Y, Ohtsuka A
  • Journal Title: Int J Biochem Cell Biol Volume: 45(5) Pages: 1003-11
  • DOI: 10.1016/j.biocel.2013.02.001
  • Peer Reviewed
• [Journal Article] A new model of development of the mammalian ovary and follicles (2013)
  • Author(s): Hummitzsch K, Irving-Rodgers HF, Hatzirodos N, Bonner W, Sabatier L, Reinhardt DP, Sado Y, Ninomiya Y, Wilhelm D, Rodgers RJ
  • Journal Title: PLoS One Volume: 8(2) Pages: e55578
  • DOI: 10.1371/journal.pone.0055578
  • Peer Reviewed
• [Journal Article] Different sizes of centrilobular ground-glass opacities in chest high-resolution computed tomography of patients with pulmonary veno-occlusive disease and patients with pulmonary capillary hemangiomatosis (2013)
  • Author(s): Miura A, Akagi S, Nakamura K, Ohta-Ogo K, Hashimoto K, Nagase S, Kohno K, Kusano K, Ogawa A, Matsubara H, Toyooka S, Oto T, Ohtsuka A, Ohe T, Ito H
  • Journal Title: Cardiovasc Pathol Volume: 22 Pages: 287-293
  • DOI: 10.1016/j.carpath.2012.12.002
  • Peer Reviewed
• [Journal Article] Architecture of the subendothelial elastic fibers of small blood vessels and variations in vascular type and size (2013)
  • Author(s): Shinaoka A, Momota R, Shiratsuchi E, Kosaka M, Kumagishi K, Nakahara R, Naito I, Ohtsuka A
  • Journal Title: Microsc Microanal Volume: 19(2) Pages: 406-14
  • DOI: 10.1017/S1431927612014341
  • Peer Reviewed
• [Journal Article] Differential expression of basement membrane type IV collagen α2 and α6 chains as a prognostic factor in patients with extrahepatic bile duct carcinoma (2013)
  • Author(s): Hirashima K, Iyama K, Baba Y, Honda Y, Sado Y, Ninomiya Y, Watanabe M, Takamori H, Beppu T, Baba H
  • Journal Title: J Surg Oncol Volume: 107(4) Pages: 402-7
  • DOI: 10.1002/jso.23225
  • Peer Reviewed
• [Journal Article] Tumor growth inhibitory effect of ADAMTS1 is accompanied by the inhibition of tumor angiogenesis (2012)
  • Author(s): Obika M, Ogawa H, Takahashi K, Li J, Hatipoglu OF, Cilek MZ, Miyoshi T, Inagaki J, Ohtsuki T, Kusachi S, Ninomiya Y, Hirohata S
  • Journal Title: Cancer Sci Volume: 103(10) Pages: 1889-97
  • DOI: 10.1111/j.1349-7006.2012.02381.x
  • Peer Reviewed
• [Journal Article] Bral2 is indispensable for the proper localization of brevican and the structural integrity of the perineuronal net in the brainstem and cerebellum (2012)
  • Author(s): Bekku Y, Saito M, Moser M, Fuchigami M, Maehara A, Nakayama M, Kusachi S, Ninomiya Y, Oohashi T
  • Journal Title: J Comp Neurol Volume: 520(8) Pages: 1721-36
  • DOI: 10.1002/cne.23009
  • Peer Reviewed
• [Journal Article] Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells (2012)
  • Author(s): Kanazawa T, Furumatsu T, Hachioji M, Oohashi T, Ninomiya Y, Ozaki T
  • Journal Title: J Orthop Res Volume: 30(3) Pages: 468-74
  • DOI: 10.1002/jor.21528
  • Peer Reviewed
• [Journal Article] Effects of pharyngeal cooling on brain temperature in primates and humans : a study for proof of principle (2012)
  • Author(s): Takeda Y, Hashimoto H, Fumoto K, Danura T, Naito H, Morimoto N, Katayama H, Fushimi S, Matsukawa A, Ohtsuka A, Morita K
  • Journal Title: Anesthesiology Volume: 117(1) Pages: 117-125
  • DOI: 10.1097/ALN.0b013e3182580536
  • Peer Reviewed
• [Journal Article] Abnormalities in the fiber composition and capillary architecture in the soleus muscle of type 2 diabetic Goto-Kakizaki rats (2012)
  • Author(s): Murakami S, Fujita N, Kondo H, Takeda I, Momota R, Ohtsuka A, Fujino H
  • Journal Title: Scientific World Journal Volume: Art.ID 680189
  • DOI: 10.1100/2012/680189
  • Peer Reviewed
• [Journal Article] Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy (2011)
  • Author(s): Minematsu H, Otani T, Oohashi T, Hirai M, Oie K, Igarashi K, Ohtsuka A
  • Journal Title: J Electron Microsc (Tokyo) Volume: 60(1) Pages: 95-99
  • DOI: 10.1093/jmicro/dfq071
  • Peer Reviewed
• [Journal Article] Anti-high mobility group box-1 monoclonal antibody protects the blood-brain barrier from ischemia-induced disruption in rats (2011)
  • Author(s): Zhang J, Takahashi HK, Liu K, Wake H, Liu R, Maruo T, Date I, Yoshino T, Ohtsuka A, Mori S, Nishibori M
  • Journal Title: Stroke Volume: 42(5) Pages: 1420-1428
  • DOI: 10.1161/STROKEAHA.110.598334
  • Peer Reviewed
• [Journal Article] Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution (2011)
  • Author(s): Momota R, Naito I, Ninomiya Y, Ohtsuka A
  • Journal Title: Matrix Biol Volume: 30 Pages: 258-266
  • DOI: 10.1016/j.matbio.2011.03.00
  • Peer Reviewed
• [Journal Article] Clonal overgrowth of esophageal smooth muscle cells in diffuse leiomyomatosis-Alport syndrome caused by partial deletion in COL4A5 and COL4A6 genes (2011)
  • Author(s): Oohashi T, Naito I, Ueki Y, Yamatsuji T, Permpoon R, Tanaka N, Naomoto Y, Ninomiya Y
  • Journal Title: Matrix Biol Volume: 30(1) Pages: 3-8
  • DOI: 10.1016/j.matbio.2010.09.003
  • Peer Reviewed
• [Journal Article] Distribution of α(IV) collagen chains in the ocular anterior segments of adult mice (2011)
  • Author(s): Saito K, Yonezawa T, Minaguchi J, Kurosaki M, Suetsugu S, Nakajima A, Nomoto H, Morizane Y, Sado Y, Sugimoto M, Kusachi S, Ninomiya Y
  • Journal Title: Connect Tissue Res Volume: 52(2) Pages: 147-56
  • DOI: 10.3109/03008207.2010.492062
  • Peer Reviewed
• [Journal Article] Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane (2011)
  • Author(s): Kinnunen AI, Sormunen R, Elamaa H, Seppinen L, Miller RT, Ninomiya Y, Janmey PA, Pihlajaniemi T
  • Journal Title: J Biol Chem Volume: 286(10) Pages: 7755-64
  • DOI: 10.1074/jbc.M110.166132
  • Peer Reviewed
• [Presentation] リジンオリゴマープローブからなる間接軟骨特異的X 線プローブの創出 (2014)
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  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 京都府医師会館
  • Year and Date: 20140228-0301
• [Presentation] マウス聴覚内側台形体核(MNTB)におけるペリニューロナルネット形成 (2013)
  • Author(s): 大橋俊孝ら
  • Organizer: MBSJ2013
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20131203-06
• [Presentation] Drosopila multiplexin mutants manifest ecdysone deficiency (2013)
  • Author(s): 百田龍輔ら
  • Organizer: Pan Pacific Connective Tissue Societies Symposium
  • Place of Presentation: Hong Kong Academy of Medicine Jockey Club Building
  • Year and Date: 20131124-27
• [Presentation] 脊髄損傷におけるIV型コラーゲンα鎖の発現と分布の継時的変化 (2013)
  • Author(s): 米澤朋子ら
  • Organizer: 日本結合組織学会・マトリックス研究会
  • Place of Presentation: 和歌山県立医科大学
  • Year and Date: 20130628-29
• [Presentation] 致死となるマルチプレキシン(XV/XVIII 型コラーゲン)変異ショウジョウバエの解析 (2013)
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  • Organizer: 日本結合組織学会・マトリックス研究会
  • Place of Presentation: 和歌山県立医科大学
  • Year and Date: 20130628-29
• [Presentation] The inhibitory role of an angiotensin II converting enzyme inhibitor on gelatinase activity in experimental abdominalaortic aneurysm (2012)
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  • Organizer: 日本血管生物医学会
  • Place of Presentation: あわぎんホール
  • Year and Date: 20121205-07
• [Presentation] The expression of interleukin-6 is attenuated by type IV collagen in cultured astrocytes (2012)
  • Author(s): 米澤朋子ら
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 20120918-21
• [Presentation] アストロサイトのサイトカイン産生におけるIV型コラーゲンの役割 (2012)
  • Author(s): 堀田輝ら
  • Organizer: 日本結合組織学会・マトリックス研究会
  • Place of Presentation: 日本青年館ホテル
  • Year and Date: 20120607-08
• [Presentation] マウス眼におけるIV型コラーゲンα鎖の分布 (2011)
  • Author(s): 斎藤健司ら
  • Organizer: 日本結合組織学会・マトリックス研究会
  • Place of Presentation: 別府ビーコンプラザ
  • Year and Date: 20110610-11
• [Book] 先天代謝異常症候群 (2012)
  • Author(s): 米澤朋子, 二宮善文
  • Total Pages: 4(683-686)
  • Publisher: 日本臨床
• [Book] 先天代謝異常症候群 (2012)
  • Author(s): 大橋俊孝ら
  • Total Pages: 5(700-704)
  • Publisher: 日本臨床
• [Book] 血管生物医学事典 (2011)
  • Author(s): 二宮善文, 他
  • Total Pages: 2(53-55)
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.mbb-okayama.com/