2013 Fiscal Year Final Research Report
Elucidation of reproductive endocrine system in hagfish
| Project/Area Number |
23570072
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Niigata University |
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Principal Investigator |
NOZAKI Masumi 新潟大学, 自然科学系, 教授 (70136232)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ヌタウナギ / 無顎類 / 下垂体 / 生殖腺刺激ホルモン / 性ステロイドホルモン / コレステロール側鎖切断酵素 / ステロイド産生細胞 |
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| Research Abstract |
Since hagfishes are considered the most primitive vertebrate known, living or extinct, studies on their reproduction are important for understanding the evolutionary aspects of the vertebrate reproductive endocrine system. Recently, single functional GTH was identified in the hagfish pituitary gland. In the present study, cytochrome P450 side-chain cleavage enzyme (CYP11A) was cloned from the hagfish testis. A clear positive correlation was noted between CYP11A mRNA levels and gonadal development in both sexes. Moreover, hagfish GTH stimulated CYP11A mRNA levels in the cultured testis. A PQRFamide peptide identified from the hagfish hypothalamus was shown to stimulate GTH mRNA levels in the hagfish pituitary. The present study further showed the presence of a steroid (estradiol) feedback system at the hypothalamic-pituitary levels. It is suggested that vertebrates, during their early evolution, have established the hypothalamic-pituitary-gonadal axis.
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[Journal Article] Relationships between plasma concentrations of sex steroid hormones and gonadal development in the brown hagfish, Paramyxine atami2013
Author(s)
Nishiyama, M., Chiba, H., Uchida, K., Shimotani, T., Nozaki, M.
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Journal Title
Zool. Sci.
Volume: 30
Pages: 967-974
DOI
Peer Reviewed
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[Journal Article] Effects of estradiol or testosterone treatment on expression of gonadotropin subunit mRNAs and proteins in the pituitary of juvenile brown hagfish, Paramyxine atami2013
Author(s)
Nozaki, M., Uchida, K., Honda, K., Shimotani, T., Nishiyama, M.
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Journal Title
Gen. Comp. Endocrinol.
Volume: 189
Pages: 111-118
DOI
Peer Reviewed
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[Journal Article] Evolutionary origin of the structure and function of gonadotropin-inhibitory hormone : insights from lampreys2012
Author(s)
Osugi, T., Daukss, D., Gazda, K., Ubuka, T., Kosugi, T., Nozaki, M., Sower, S.A., Tsutsui, K.
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Journal Title
Endocrinology
Volume: 153
Pages: 2362-2374
DOI
Peer Reviewed
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[Presentation] Study on sex steroid hormones in the hagfish - Their serum concentrations and sex steroid biosynthetic enzymes2012
Author(s)
Nishiyama, M., Uchida, K., Moriyama, S., Chiba, H., Awata, S., Nozaki, M.
Organizer
The 7th Asia and Oceania Society for Comparative Endocrinology
Place of Presentation
Kuala Lumpur, Malaysia
Year and Date
20120303-07
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