Analyses of the mechanism of broad-spectrum disease resistance by BSR1 in rice

2013 Fiscal Year Final Research Report

• Project/Area Number: 23580142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: MASAKI Mori 独立行政法人農業生物資源研究所, 耐病性作物研究開発ユニット, 上級研究員 (50192779)
• Project Period (FY): 2011 – 2013
• Keywords: 病害抵抗性 / イネ

Research Abstract

Overexpression of BSR1, a novel kinase gene, confers broad-spectrum disease resistance against bacterial and fungal pathogens in rice. Microarray and genetic analyses uncovered that the resistance is largely independent of the salicylic acid pathway. Loss-of-function analyses showed that BSR1 is involved in innate immunity of rice. Overexpression of BSR1 conferred resistance against two more diseases in addition to leaf blight and blast in rice. Further, overexpression of BSR1 conferred resistance to bacterial speck in dicot tomato.

Research Products

• [Journal Article] High-throughput analysis of rice genes by means of the heterologous full-length cDNA overexpressor (FOX)-hunting system (2013)
  • Author(s): Higuchi-Takeuchi M, Mori M, Matsui M.
  • Journal Title: The International Journal of Developmental Biology Volume: 57(6/7/8) Pages: 517-523
  • DOI: 10.1387/ijdb.130176mm
  • Peer Reviewed
• [Journal Article] 広範な植物病原菌に対する抵抗性遺伝子-イネからBSR1 遺伝子をFOX ハンティングにより発見- (2012)
  • Author(s): 前田哲, 森昌樹
  • Journal Title: 化学と生物 Volume: 50(9) Pages: 627-628
  • Peer Reviewed
• [Journal Article] Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice (2011)
  • Author(s): Dubouzet J.G, Maeda S, Sugano S, Ohtake M, Hayashi N, Ichikawa T, Kondou Y, Kuroda H, Horii Y, Matsui M, Oda K, Hirochika H, Takatsuji H, Mori M.
  • Journal Title: Plant Biotechnology Journal Volume: 9(4) Pages: 466-485
  • DOI: 10.1111/j.1467-7652.2010.00568.x
  • Peer Reviewed
• [Presentation] Broad-spectrum disease resistance by overexpression of rice BSR1 and its application to crop improvement (2013)
  • Author(s): Mori M, Maeda S, Sugano S, Naoki Y, Hayashi N, Goto S, Jiang C-J, Oda K, Hirochika H, Takatsuji H.
  • Organizer: 11th International Symposium on Rice Functional Genomics
  • Place of Presentation: New delhi
  • Year and Date: 20131120-23
  • Invited
• [Presentation] イネの複合病害抵抗性遺伝子BSR1 による非常に広範な病害に対する抵抗性の付与 (2013)
  • Author(s): 前田哲, 林長生, 森昌樹
  • Organizer: 日本育種学会124回講演会
  • Place of Presentation: 鹿児島
  • Year and Date: 20131012-13
• [Presentation] A cytoplasmic kinase gene provides resistance against major bacterial and fungal pathogens in Arabidopsis and rice (2013)
  • Author(s): Maeda S, Sugano S, Dubouzet J.G, Yokotani N, Jiang C.J, Oda K, Matsui M, Hirochika H, Takatsuji H, Mori M.
  • Organizer: 10th International Congress of Plant Pathology
  • Place of Presentation: Beijin
  • Year and Date: 20130825-30
  • Invited
• [Presentation] イネの複合病害抵抗性遺伝子BSR1 による抵抗性にはサリチル酸非依存性の経路が主に関与する (2013)
  • Author(s): 前田哲, 菅野正治, 中込マリコ, 宮尾安藝雄, 姜昌杰, 西澤洋子, 高辻博志, 森昌樹
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] イネの複合病害抵抗性遺伝子BSR1 とサリチル酸シグナルとの関連解析 (2012)
  • Author(s): 前田哲, 菅野正治, 中込マリコ, 宮尾安藝雄, 姜昌杰, 高辻博志, 森昌樹
  • Organizer: 日本育種学会122回講演会
  • Place of Presentation: 京都
  • Year and Date: 20120914-15
• [Presentation] Broad-spectrum disease resistance by BSR1 shares transcriptional components with BTH-inducible resistance in rice (2012)
  • Author(s): Maeda S, Sugano S, Nakagome M, Miyao A, Jiang C-J, Takatsuji H, Mori M.
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都
  • Year and Date: 20120729-0802
• [Presentation] 広範な病害に対する抵抗性遺伝子BSR1 による抵抗性機構のマイクロアレイ解析 (2012)
  • Author(s): 前田哲, 菅野正治, 高辻博志, 森昌樹
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] Identification of BSR1 gene that confers resistance to rice blight and blast by fox hunting (2011)
  • Author(s): Maeda S, Dubouzet J.G, Sugano S, Ichikawa T, Kondou Y, Yokotani N, Oda K, Matsui M, Hirochika H, Takatsuji H, Mori M.
  • Organizer: 9th International Symposium of Rice Functional Genomics
  • Place of Presentation: Taipei
  • Year and Date: 20111107-06
  • Invited
• [Presentation] 複合病害抵抗性遺伝子BSR1 による単子葉および双子葉作物への病害抵抗性の付与 (2011)
  • Author(s): 前田哲, 横谷尚起, 菅野正治, 小田賢司, 松井南, 廣近洋彦, 高辻博志, 森昌樹
  • Organizer: 日本育種学会120回講演会
  • Place of Presentation: 福井
  • Year and Date: 20110923-24
• [Book] Characterization of rice genes using a heterologous full-length cDNA expression system Transgenic Plants: Methods and Protocols 3(8) (2012)
  • Author(s): Higuchi M, Kondou Y, Mori M, Ichikawa T, Matsui M.
  • Total Pages: 75-90
  • Publisher: Springer
• [Remarks] 日経バイオテク2011.9.26 日号 p.17-18 で学会発表⑨の内容について紹介される。
• [Remarks] ① 森昌樹 "万が一"の発見!～病気に強く、環境にやさしい作物をつくる～ 平成24 年度農業生物資源研究所一般公開サイエンスカフェ 2012.4.21、つくば(アウトリーチ活動)
• [Remarks] ② 森昌樹 広範な植物病原菌に対する抵抗性遺伝子～分子育種への利用～ 第10 回バイオテクノロジー国際会議, バイオアカデミックフォーラム 2011.6.29、東京(アウトリーチ活動)
• [Patent(Industrial Property Rights)] リゾクトニア菌抵抗性遺伝子 (2014)
  • Inventor(s): 森昌樹、前田哲、横谷尚起、小田賢司、近藤陽一、松井南
  • Industrial Property Rights Holder: 森昌樹、前田哲、横谷尚起、小田賢司、近藤陽一、松井南
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2014/052684
  • Filing Date: 2014-02-05
  • Overseas
• [Patent(Industrial Property Rights)] リゾクトニア菌抵抗性遺伝子 (2013)
  • Inventor(s): 森昌樹、前田哲、横谷尚起、小田賢司、近藤陽一、松井南
  • Industrial Property Rights Holder: 森昌樹、前田哲、横谷尚起、小田賢司、近藤陽一、松井南
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2013-020066
  • Filing Date: 2013-02-05
  • Overseas