2013 Fiscal Year Final Research Report
Whole cell-dependent production of polyphenol conjugates using genetically engineered budding yeast
| Project/Area Number |
23580179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAKI Toshiyuki 富山県立大学, 工学部, 教授 (70293909)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 機能性成分 / ポリフェノール / グルクロン酸抱合 / 硫酸抱合 |
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| Research Abstract |
In order to synthesize the conjugates as dietary metabolites, polyphenols, we have developed several mammalian UDP-glucuronosyltransferases (UGT) or sulfotransferase (SULT) expression systems in budding yeast. For the glucuronide production, mammalian UGT and UDP-glucose dehydrogenase (UGDH) were expressed in budding yeas using a multicopy and a genome integrated vectors. Using genetically engineered yeast containing UGT and UGDH, glucuronide formation of quercetin was examined. Querectin with multiple glucuronidating sites was conjugated as isoform-dependent formation using UGT isoforms. In the presence of glucose and ammonium sulfate, formation of sulfated quercetin was observed in whole-cell production system with SULT isoforms .These expression system of mammalian UGT or SULT in budding yeast would be a powerful tool for enzyme-assisted synthesis of various dietary metabolites including glucuronides and sulfates.
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[Journal Article] Multiple isoform dependent glucuronidation of a selective c-Fos/activator protein-1 inhibitor T-52242011
Author(s)
Uchihashi, S.,Fukumoto, H., Onoda, M., Hayakawa, H., Ikushiro, S., and Sakaki, T.
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Journal Title
Drug Metabolism and Disposition
Volume: 39
Pages: 803-813
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