2013 Fiscal Year Final Research Report
Ultrastructural studies on the intracellular trafficking of membranes and HCl-secreting proteins in gastric parietal cells by high-pressure freezing.
| Project/Area Number |
23590238
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 高圧凍結技法 / 胃粘膜 / 胃酸分泌 / 壁細胞 / 超微形態 / 細胞膜動態 / 免疫組織化学 / 蛋白局在 |
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| Research Abstract |
We investigated the intracellular trafficking of membranes and HCl-screting proteins in rat gastric parietal cells by taking advantages of the ex vivo experimental model, named rat isolated gastric mucosa, and the high-pressure freezing followed by freeze substitution cryo-techniques.
As results, we obtained a new insight concerning the intracellular trafficking of membrane fusion proteins, such as syntaxin3 and Hip1r, and ion-transporter channel proteins, such as KCNQ1, between the resting state and the active acid-secreting state of the parietal cell by the application of immuno-histochemistry. In addition, we revealed a unique change in functional morphology in the gastric parietal cells of TRIM50 knock-out mice at the electron microscopical level.
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[Journal Article] TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells2012
Author(s)
Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Van, B., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A., Takeshima, H
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Journal Title
J of Biol Chem
Volume: 287(40)
Pages: 33523-32
Peer Reviewed
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