Analysis of LECT2 receptor and its signal transduction

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590275
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SATOSHI Yamagoe 国立感染症研究所, 真菌部, 主任研究官 (00212283)
• Project Period (FY): 2011 – 2013
• Keywords: 生理活性 / シグナル伝達 / LECT2

Research Abstract

Accumulating evidences indicate that LECT2 is associated with several physiological phenomena, but its function is not fully understood. To make clear the function of LECT2, it is important to identify the LECT2 receptor. We performed expression cloning with a retroviral cDNA library constructed from a cell line that highly expressed the LECT2 receptor. Retrovirus infected cells binding LECT2-Fc fusion protein were enriched by cell sorting. The seven cell lines cloned from the enriched cells all had cDNAs derived from the same gene in integrated retrovirus vector. We checked the possibility that this protein was the LECT2 receptor by some experiments. Moreover, we analyzed the phosphoprotein involved in LECT2 signal transduction by western blotting with thirty different kinds of anti-phosphoprotein antibodies, identifying some possible phosphoproteins.

Research Products

• [Journal Article] Increased serum leukocyte cell-derived chemotaxin 2 (LECT2) levels in obesity and fatty liver (2013)
  • Author(s): Akinori Okumura, Hiroyuki Unoki-Kubota, Yumi Matsushita, Tomoko Shiga, Yuriko Moriyoshi, Satoshi Yamagoe, Yasushi Kaburagi
  • Journal Title: BioScience Trends Volume: 7 Pages: 276-283
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of human leukocyte cell-derived Chemotaxin 2 (LECT2) (2013)
  • Author(s): Hai Zheng, Takuya Miyakawa, Yoriko Sawano, Satoshi Yamagoe, Masaru Tanokura
  • Journal Title: Acta Crystallographica Section F Volume: 63 Pages: 316-319
  • DOI: 10.1107/S1744309113003758
  • Peer Reviewed
• [Journal Article] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein (2013)
  • Author(s): Akinori Okumura, Takehiro Suzuki, Hideyuki Miyatake, Tomoya Okabe, Yuki Hashimoto, Takuya Miyakawa, HaiZheng, Hiroyuki Unoki-Kubota, Hideaki Ohno, NaoshiDohmae, Yasushi Kaburagi, Yoshitsugu Miyazaki, Masaru Tanokura, Satoshi Yamagoe
  • Journal Title: FEBS letter Volume: 587 Pages: 404-409
  • DOI: 10.1016/j.febslet.2013.01.025
  • Peer Reviewed
• [Journal Article] Expression, high-pressure refolding and purification of human leukocyte cell-derived chemotaxin 2 (LECT2) (2013)
  • Author(s): Hai Zheng, Takuya Miyakawa, Yoriko Sawano, Satoshi Yamagoe, Masaru Tanokura
  • Journal Title: Protein expression and purification Volume: 88 Pages: 221-229
  • DOI: 10.1016/j.pep.2013.01.008
  • Peer Reviewed
• [Journal Article] LECT2 Functions as a Hepatokine That Links Obesity to Skeletal Muscle Insulin Resistance (2012)
  • Author(s): Fei Lan, Hirofumi Misu, Keita Chikamoto, Hiroaki Takayama, Akihiro Kikuchi, Kensuke Mohri, Noboru Takata, Hiroto Hayashi, Naoto Matsuzawa-Nagata, Yumie Takeshita, Hiroyo Noda, Yukako Matsumoto, Tsuguhito Ota, Toru Nagano, Masatoshi Nakagen, Ken-ichi Miyamoto, Kanako Takatsuki, Toru Seo, Kaito Iwayama, Kunpei Tokuyama, Seiichi Matsugo, Hong Tang, Yoshiro Saito, Satoshi Yamagoe, Shuichi Kaneko, Toshinari Takamura
  • Journal Title: Diabetes Volume: 63 Pages: 1649-1664
  • DOI: 10.2337/db13-0728
  • Peer Reviewed
• [Journal Article] Oncogenicβ-catenin triggers an inflammatory response that determines the aggressiveness of hepatocellular carcinoma in mice (2012)
  • Author(s): Marie Anson, Anne-Marie Crain-Denoyelle, Véronique Baud, Fanny Chereau, AngéliqueGougelet, Benoit Terris, Satoshi Yamagoe, Sabine Colnot, Mireille Viguier, Christine Perret, Jean-Pierre Couty
  • Journal Title: Journal Clinical Investigation Volume: 122 Pages: 586-599
  • DOI: 10.1172/JCI43937
  • Peer Reviewed
• [Presentation] Leukocyte cell-derived chemotaxin 2 is a zinc-binding protein : the monomeric forms are stabilized by the zinc ion (2013)
  • Author(s): Akinori Okumura, Takehiro Suzuki, Hideyuki Miyatake, Takuya Miyakawa, HaiZheng, Hiroyuki Unoki-Kubota, NaoshiDohmae, Yasushi Kaburagi, Masaru Tanokura, Satoshi Yamagoe
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20131200
• [Presentation] Hepatokine LECT2 causes insulin resistance in skeletal muscle throught increased JNK phosphorylation (2013)
  • Author(s): Fei Lan, Hirofumi Misu, Keita Chikamoto, Hiroaki Takayama, Akihiro Kikuchi, Satoshi Yamagoe, Shuichi Kaneko, Toshinari Takamura
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 20131200
• [Remarks] ホームページ
  • URL: http://www.geocities.jp/lect2_yamagoe/