Analysis of Oncogene-induced-Senescence related genes in cancer for clinical application

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591986
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nagoya University
• Principal Investigator: IGAMI TSUYOSHI 名古屋大学, 医学部附属病院, 病院講師 (50420378)
• Co-Investigator(Kenkyū-buntansha): NAGINO Masato 名古屋大学, 医学系研究科, 教授 (20237564) ; YOKOYAMA Yukihiro 名古屋大学, 医学部附属病院, 講師 (80378091) ; KOKURYO Toshio 名古屋大学, 医学系研究科, 特任講師 (60378023)
• Project Period (FY): 2011 – 2013
• Keywords: Senescence / HJURP

Research Abstract

Senescence is the irreversible cell cycle arrest for the shortage of telomea. We identified gene HJURP (Holliday junction recognition protein) as Oncogene induced Senescence connection gene. HJURP is one kind of the histone chaperon. HJURP is highly expressed in human cholangiocarcinoma cell strain and pancreatic cancer cell strain. HJURP siRNA suppressed the cell proliferation in cholangiocarcinoma cell strain. In addition, we examined the combination treatment of anticancer agent CDDP and HJURP siRNA. The cell proliferation was inhibited in HJURP siRNA2 group, compared to control siRNA group. HJURP siRNA2 enhanced the effect of anticancer agent CDDP.
Next, the prognosis is poor in the overexpression group of HJURP in cholangiocarcinoma and pancreatic cancer. Our data suggested that HJURP may be a prognostic prediction marker in the pancreatic cancer and cholangiocarcinoma.