The plakin family proteins can be therapeutic targets for intrahepatic cholangiocarcinoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: UYAMA Naoki 兵庫医科大学, 医学部, 講師 (70402873)
• Co-Investigator(Kenkyū-buntansha): FUJIMOTO Jiro 兵庫医科大学, 医学部, 教授 (90199373) ; IIMURO Yuji 兵庫医科大学, 医学部, 教授 (30252018) ; HIRANO Tadamichi 兵庫医科大学, 医学部, 准教授 (90340968) ; SATAKE Makoto 兵庫医科大学, 医学部, 助教 (70399153)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 肝内胆管癌 / Plakin / 癌細胞 / 線維芽細胞

Outline of Final Research Achievements

Plakin Family consists of cytoskeletal proteins such as plectin , desmoplakin and so on. In this project, we investigated expression of plectin and desmoplakin in human intrahepatic cholangiocarcinoma (ICC) and their fuctions. Immunohistochemistry revealed that these proteins were expressed by cancer cells as well as fibroblasts. In addition, integrin alpha6 and beta4, binding proteins of plectin, and desmocollin desmoglein, binding proteins of desmoplakin, were also expressed by cancer cells. Gene depletion experiments with SiRNA for these proteins revealed that they did not regulate ICC celllines function, such as proliferaion ability, migration ability and ability for activating fibroblas.

Free Research Field

肝内胆管癌