Functional analysis of CCN2 as a metabolic supporter and potential clinical applications in chondrocyte regeneration

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592216
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: MIYAKE Yoshiaki 岡山大学, 医学部, 客員研究員 (70594810)
• Co-Investigator(Kenkyū-buntansha): AOYAMA Eriko 岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650) ; FURUMATSU Takayuki 岡山大学, 岡山大学病院, 助教 (20432651) ; KUBOTA Satoshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (90221936) ; OZAKI Toshifumi 岡山大学, 大学院医歯薬学総合研究科, 教授 (40294459) ; TAKIGAWA Masaharu 岡山大学, 大学院医歯薬学総合研究科, 教授 (20112063)
• Project Period (FY): 2011 – 2013
• Keywords: CCN2 / 変形性関節症 / メタボローム解析

Research Abstract

Functional analysis and potential clinical applications of CCN2 as a metabolic supporter in chondrocyte regeneration. The purpose of this study was to analyze the influence of connective tissue growth factor (CCN2) on overall metabolism in chondrocytes, especially energy production and a molecular basis related to the energy production. The result revealed that the absence of CCN2 caused the decrease in the amount of adenosine triphosphate (ATP) in chondrocytes and suppression of glycolysis. Also, CCN2 was shown to be a metabolic supporter in chondrocyte regeneration producing ATP. Furthermore, the effect of articular cartilage regeneration in an osteoarthritis (OA) model suggested future clinical application for osteoarthritis cartilage treatment.

Research Products

• [Journal Article] CCN2 as a novel molecule supporting energy metabolism of chondrocytes (2014)
  • Author(s): Maeda-Uematsu, A, Kubota, S., Kawaki, H., Kawata, K., Miyake, Y., Hattori, T., Nishida, T., Moritani, N., Lyons, K.M., Iida, S. and Takigawa. M
  • Journal Title: J Cell Biochem Volume: 115 Pages: 854-865
  • Peer Reviewed
• [Presentation] 軟骨細胞のエネルギー代謝におけるCCN2の役割 (2014)
  • Author(s): 前田彩、久保田聡、川木晴美、河田かずみ、三宅由晃、服部高子、西田崇、森谷徳文、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都
  • Year and Date: 20140228-0301
• [Presentation] Crucial role of CCN2 in energy metabolism in chondrocytes (2013)
  • Author(s): Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Hattori, T., Nishida, T., Moritani, N., Kawaki, H., Lyons, K., Iida, S., Takigawa, M.
  • Organizer: 2nd joint meeting of IBMS-JSBMR
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20130528-0601
• [Presentation] 軟骨細胞のエネルギー代謝を支えるCCN2/CTGF (2013)
  • Author(s): 前田彩、久保田聡、三宅由晃、河田かずみ、西田崇、服部高子、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 20130301-02
• [Presentation] CCN2は軟骨細胞のエネルギー代謝に重要である (2013)
  • Author(s): 前田彩、久保田聡、川木晴美、河田かずみ、三宅由晃、服部高子、西田崇、森谷徳文、飯田征二、滝川正春
  • Organizer: 第5回日本CCNファミリー研究会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-20
• [Presentation] Roles of CCN2 in energy metabolism in chondrocytes (2012)
  • Author(s): Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Nishida, T., Hattori, T., Moritani, N., Kawaki, H., Lyons, K.M., Iida, S., Takigawa, M.
  • Organizer: 2012 The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, California
  • Year and Date: 20121215-19
• [Presentation] 軟骨細胞のエネルギー代謝におけるCCN2/CTGFの役割 (2012)
  • Author(s): 前田彩、久保田聡、三宅由晃、河田かずみ、西田崇、服部高子、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] CCN2/CTGF欠損が軟骨細胞のエネルギー代謝に及ぼす影響 (2012)
  • Author(s): 前田彩、久保田聡、服部高子、西田崇、飯田征二、滝川正春
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
  • Year and Date: 20120914-16
• [Presentation] 軟骨細胞の基本代謝におけるCCN2の役割 (2012)
  • Author(s): 前田彩、久保田聡、三宅由晃、河田かずみ、森谷徳文、滝川正春
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 20120719-21
• [Presentation] 軟骨細胞の代謝システムにおけるCCN2の役割 (2012)
  • Author(s): 前田彩、久保田聡、三宅由晃、河田かずみ、服部高子、西田崇、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第33回岡山歯学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2012-11-25