Identification and functional analysis of microRNAs involved in the onset of proteinurea accompanied with pregnancy-induced hypertension

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka Prefecture University (2012-2013); Nippon Medical School (2011)
• Principal Investigator: ISHIBASHI OSAMU 大阪府立大学, 生命環境科学研究科(系), 准教授 (70293214)
• Project Period (FY): 2011 – 2013
• Keywords: 妊娠高血圧症候群 / ポドサイト / マイクロRNA / 次世代シーケンス / 蛋白尿

Research Abstract

This study was conducted in order to elucidate the molecular mechanism of proteinurea onset accompanied with pregnancy-induced hypertension (PIH). Primary podocytes (glomerular epithelial cells) were treated with endothelin-1 to induce cellular injury to the cells, whereby an in vitro pathological model reflecting the PIH-related proteinurea was established. Further, transcripts (including non-coding RNAs) dysregulated in accordance with the podocyte injury were explored in a genome-wide fashion by means of next-generation sequencing and PCR-based microRNA array system.

Research Products

• [Journal Article] Dataset of microarray analysis to identify endoglin-dependent bone morphogenetic protein-2-responsive genes in the murine periodontal ligament cell line PDL-L2 (2014)
  • Author(s): Ishibashi, O., Inui, T
  • Journal Title: Genomics Data Volume: 2巻 Pages: 24-26
  • Peer Reviewed
• [Journal Article] Interactions between the non-seed region of siRNA and RNA-binding RLC/RISC proteins, Ago and TRBP, in mammalian cells (2014)
  • Author(s): Takahashi, T., Zenno, S., Ishibashi, O., Takizawa, T., Saigo, K., Ui-Tei, K
  • Journal Title: Nucleic Acids Res Volume: 42巻 Pages: 5256-5269
  • Peer Reviewed
• [Journal Article] BMP-2 desensitizes MC3T3-E1 osteoblastic cells to estrogen through transcriptional downregulation of estrogen receptor 1 (2013)
  • Author(s): Ishibashi, O
  • Journal Title: J. Bone Metab Volume: 20巻 Pages: 83-88
  • Peer Reviewed
• [Journal Article] Plasma level of hydroxysteroid (17-β) dehydrogenase 1 in the second trimester is an independent risk factor for predicting preeclampsia after adjusting for the effects of mean blood pressure, bilateral notching and plasma level of soluble fms-like tyrosine kinase 1/placental growth factor ratio (2012)
  • Author(s): Ohkuchi, A., Ishibashi, O., Hirashima, C., Takahashi, K., Matsubara, S., Takizawa, T., Suzuki, M. (共筆頭著者)
  • Journal Title: Hypertens. Res Volume: 35巻 Pages: 1152-1158
  • Peer Reviewed
• [Journal Article] MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines (2012)
  • Author(s): Mase, Y., Ishibashi, O., Ishikawa, T., Takizawa, T., Kiguchi, T., Ohba, T., Katabuchi, H., Takeshita, T., Takizawa, T
  • Journal Title: Reprod. Sci. Volume: 19巻 Pages: 1030-1040
  • Peer Reviewed
• [Journal Article] Hydroxysteroid (17-beta) dehydrogenase 1 is dysregulated by mir-210 and mir-518c that are aberrantly expressed in preeclamptic placentas : A novel marker for predicting preeclampsia (2012)
  • Author(s): Ishibashi, O., Ohkuchi, A., Ali, M.M., Kurashina, R., Luo, S-S., Ishikawa, T., Takizawa, T., Hirashima, C, Takahashi, K., Migita, M., Ishikawa, G., Yoneyama, K., Asakura, H., Izumi, A., Matsubara,S., Takeshita, T., Takizawa, T.
  • Journal Title: Hypertension Volume: 59巻 Pages: 265-273
  • Peer Reviewed
• [Journal Article] Short RNA duplexes elicit apoptosis via the sequential activation of PKR, RIG-I, and p38 in a cell type- and length-dependent manner (2011)
  • Author(s): Ishibashi, O., Ali, M.M., Luo, S-S., Ohba, T., Katabuchi, H., Takeshita, T., Takizawa, T.
  • Journal Title: Sci. Signal Volume: 4巻 Pages: ra74
  • Peer Reviewed
• [Journal Article] Real-time PCR-based microRNAome of human bile detects miR-9 as a potential diagnostic biomarker for biliary tract cancer (2011)
  • Author(s): Shigehara, K., Yokomuro, S., Ishibashi, O., Mizuguchi, Y., Arima, Y., Kawahigashi, Y., Kanda, T., Akagi, I., Tajiri, T., Takizawa, T., Yoshida, H., Uchida, E
  • Journal Title: PLoS ONE Volume: 6巻 Pages: e23584
  • Peer Reviewed
• [Presentation] マウス成熟破骨細胞の大規模トランスクリプトーム解析と遺伝子発現プロファイリング (2013)
  • Author(s): 小谷康斗, 他
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸(兵庫)
  • Year and Date: 2013-12-05
• [Presentation] マウス歯根膜細胞におけるBMP2に対するendoglin依存的応答遺伝子の探索 (2013)
  • Author(s): 石橋宰, 他
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 2013-09-13
• [Presentation] エクソソームmicroRNAを介した胎盤-リンパ球間コミュニケーション:培養細胞を用いたモデル解析 (2011)
  • Author(s): アリモハメド, 他
  • Organizer: 第27回日本生殖免疫学会
  • Place of Presentation: 名古屋(愛知)
  • Year and Date: 2011-12-03
• [Presentation] 妊婦血漿中でのHSD17B1の検出と妊娠高血圧腎症発症例におけるその動態 (2011)
  • Author(s): 石橋宰, 他
  • Organizer: 第32回日本妊娠高血圧学会
  • Place of Presentation: 金沢(石川)
  • Year and Date: 2011-10-22
• [Remarks]
  • URL: http://www.bioinfo.osakafu-u.ac.jp/~inuit/