2013 Fiscal Year Final Research Report
Mechanisms of tumor-induced immune tolerance during the process of peritoneal dissemination of ovarian cancer
Project/Area Number |
23592459
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
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Research Institution | Wakayama Medical University |
Principal Investigator |
INO Kazuhiko 和歌山県立医科大学, 医学部, 教授 (60303640)
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Co-Investigator(Kenkyū-buntansha) |
MABUCHI Yasushi 和歌山県立医科大学, 医学部, 助教 (80382357)
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Project Period (FY) |
2011 – 2013
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Keywords | 卵巣癌 / 腫瘍免疫 / 免疫寛容 / 腹膜播種 / マウスモデル / 酵素 |
Research Abstract |
Indoleamine 2,3-dioxygenase (IDO) is a tryptophan-catabolizing enzyme that has immunoregulatory functions. The purpose of the present study is to clarify the role of IDO during the process of peritoneal dissemination of ovarian cancer. IDO-cDNA was transfected into the murine ovarian carcinoma cell line HM-1, establishing IDO-overexpressing cells (HM-1-IDO). HM-1-IDO or mock were intraperitoneally transplanted into syngeneic immunocompetent mice. HM-1-IDO-transplanted mice showed significantly shortened survival, and increased tumor weight and ascites with the significantly reduced numbers of CD8+ T cells and NK cells within tumors as well as increased levels of TGF-beta in ascites. These data show that tumoral IDO promotes the peritoneal dissemination of ovarian cancer through suppression of tumor-infiltrating T and NK-cell recruitment and enhancement of immunosuppressive cytokines in ascites, thus IDO may be a promising molecular target for the therapeutic strategy of ovarian cancer.
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[Journal Article] Indoleamine 2,3-dioxygenase promotes peritoneal metastasis of ovarian cancer via inducing immunosuppressive environment2014
Author(s)
Tanizaki Y, Kobayashi A, Toujima S, Shiro M, Mizoguchi M, Mabuchi Y, Yagi S, Minami S, Takikawa O, Ino K
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Journal Title
DOI
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[Journal Article] Role of the renin-angiotensin system in gynecologic cancers2011
Author(s)
Ino K, Shibata K, Yamamoto E, Kajiyama H, Nawa A, Mabuchi Y, Yagi S, Minami S, Tanizaki Y, Kobayashi A, Kikkawa F
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Journal Title
Curr Cancer Drug Targets
Volume: 11(4):.
Pages: 405-11
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[Presentation] Suppression of STAT1 signal pathways reduces ovarian cancer progression2013
Author(s)
Aya Kobayashi, Yuko Tanizaki, Saori Toujima, Tamaki Yahata, Madoka Yamamoto, Michihisa Shiro, Nami Ota, Yasushi Mabuchi, Shigetaka Yagi, Sawako Minami, Toshikazu Kondo, Kazuhiko Ino
Organizer
The 3rd ASGO / The 55th JSGO
Place of Presentation
Kyoto
Year and Date
20131213-15
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[Presentation] Pivotal roles of CX3CL1-CX3CR1 axis on host cells in ovarian cancer progression2013
Author(s)
Yuko Tanizaki, Aya Kobayashi, Saori Toujima, Tamaki Yahata, Madoka Yamamoto, Michihisa Shiro, Nami Ota, Yasushi Mabuchi, Shigetaka Yagi, Sawako Minami, Toshikazu Kondo, Kazuhiko Ino
Organizer
The 3rd ASGO / The 55th JSGO
Place of Presentation
Kyoto
Year and Date
20131213-15
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