The mechanism of interaction with parathyroid hormone/parathyroid hormone-related protein receptor signals and Wnt/b-catenin in osteo-remodeling tooth migration model

2013 Fiscal Year Final Research Report

• Project/Area Number: 23593016
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: OHKUBO Kazumi 東京大学, 保健・健康推進本部, 講師 (10396715)
• Co-Investigator(Kenkyū-buntansha): NAGAHAMA Kouhei 東京大学, 医学部附属病院, 助教 (60401361) ; INOGUCHI Takato 東京大学, 医学部附属病院, 助教 (80587775) ; SAIJO Hideto 東京大学, 医学部附属病院, 講師 (80372390) ; TEI Yuichi 東京大学, 工学(系)研究科(研究院), 教授 (30345053)
• Project Period (FY): 2011 – 2013
• Keywords: 歯科矯正学 / 骨改造現象 / βカテニン / PTH受容体

Research Abstract

To investigate the underlying mechanisms of action and functional relevance of b-catenin in osteoblasts, by examining the role of b-catenin as a novel protein that interacts with the intracellular C-terminal portion of the parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor type 1 (PTHR-1). b-catenin physically interacted and co-localized with the cell membrane-specific region of PTHR-1 (584-589). Binding of b-catenin to PTHR-1 caused suppression of the Gs/cAMP pathway and enhancement of the Gq/Ca2+ pathway, without affecting the canonical Wnt pathway. Now, to analyze the mechanism of osteogenic differentiation in vivo, OsxCre:b-catenin flox mice model is treated intermittently with PTH and will be investigate these histological analysis.

Research Products

• [Journal Article] Incisor inclination after presurgical orthodontic treatment in patients with mandibular prognathism (2014)
  • Author(s): Ohkubo K, Susami T, Inokuchi T, Okayasu M, Takahashi N, Uwatoko K, Uchino N, Suenaga H, Koga Y, Saijo H, Mori Y and Takato T
  • Journal Title: Jpn J Jaw Deform Volume: 24 Pages: 16-26
  • DOI: 10.5927/jjjd.24.16
• [Journal Article] Submucous cleft palate : variations in bony defects of the hard palate (2013)
  • Author(s): Mori Y, Hoshi K , Takato T, Takahashi M , Hirano Y, Kanno Y, Ohkubo K , Saijo H
  • Journal Title: Br J Oral Maxillofac Surg Volume: 51 Pages: e220-223
  • DOI: 10.1016/j.bjoms.2013.01.015
• [Journal Article] Maxillary Protraction in Patients With Cleft Lip and Palate in Mixed Dentition : Cephalometric Evaluation After Completion of Growth (2013)
  • Author(s): Susami T, Okayasu M, Inokuchi T, Ohkubo K, Uchino N, Uwatoko K, Takahashi-Ichikawa N, Nagahama K, Takato T
  • Journal Title: Cleft Palate Craniofac J Volume: (Epub ahead of print)
  • DOI: 10.1597/12-032
• [Journal Article] Evaluation of mandibular hypoplasia in patients with hemifacial microsomia : a comparison between panoramic radiography and three-dimensional computed tomography (2013)
  • Author(s): Takahashi-Ichikawa N, Susami T, Nagahama K, Ohkubo K, Okayasu M, Uchino N, Uwatoko K, Saijo H, Mori Y, Takato T
  • Journal Title: Cleft Palate Craniofac J Volume: 50(4) Pages: 381-7
  • DOI: 10.1597/11-188
• [Journal Article] Hollow Fiber Module Applied for Effective Proliferation and Harvest of Cultured Chondrocytes (2013)
  • Author(s): Mori Y, Watanabe M, Nakagawa S, Asawa Y, Nishizawa S, Okubo K, Saijo H, Nagata S, Fujihara Y, Takato T, Hoshi K
  • Journal Title: Materials Sciences and Applications Volume: 50(4) Pages: 381-7
  • DOI: 10.4236/msa.2013.48A008
• [Journal Article] Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage (2013)
  • Author(s): Mori Y, Kanazawa S, Watanabe M, Suenaga H, Okubo K, Nagata S, Fujihara Y, Takato T, Hoshi K
  • Journal Title: Materials Sciences and Applications Volume: 4 Pages: 73-78
  • DOI: 10.4236/msa.2013.48A010
• [Journal Article] Application of percutaneous zygomatic arch osteotomy for protrusion deformity of the zygoma following facial fracture surgery (2013)
  • Author(s): Saijo H, Kawase-Koga Y, Mori Y, Seto I, Abe M, Ohkubo K, Hoshi K, Takato T
  • Journal Title: J Oral Maxillofac Surg Med Pathol Volume: 26(2) Pages: 138-141
  • DOI: 10.1016/j.ajoms.2012.09.004
• [Journal Article] 矯正歯科治療開始前の口蓋裂患者の顎顔面形態 (2013)
  • Author(s): 仲宗根愛子、須佐美隆史、内野夏子、井口隆人、岡安麻里、上床喜和子、高橋直子、大久保和美、森良之、高戸毅
  • Journal Title: 日本口蓋裂学会雑誌 Volume: 38 Pages: 113-119
  • DOI: 10.11224/cleftpalate.38.113
• [Journal Article] 東大病院における口唇口蓋裂患者に対する矯正 (2013)
  • Author(s): 須佐美隆史、大久保和美、井口隆人、岡安麻里、内野夏子、上床喜和子、高橋直子、高戸毅
  • Journal Title: 歯科治療日口蓋誌 Volume: 38巻1号 Pages: 54-61
  • DOI: 10.11224/cleftpalate.38.54
• [Journal Article] 顎顔面領域における骨軟骨再生医療の現状と展望 (2012)
  • Author(s): 高戸毅、星和人、藤原夕子、西條英人、菅野勇樹、大久保和美、鄭雄一、森良之
  • Journal Title: 頭頸部外科 Volume: 22巻2号 Pages: 121-124
  • URL: https://www.jstage.jst.go.jp/article/jjshns/22/2/22_121/_pdf
• [Journal Article] Secondary repair of an oblique facial cleft with an absorbable mesh tray and particulate cancellous bone and marrow (2012)
  • Author(s): Sugiyama M, Saijo H, Hoshi K, Ohkubo K, Mori Y, Takato T
  • Journal Title: Oral Sci Int Volume: 9(2) Pages: 63-66
  • DOI: 10.1016/S1348-8643(12)00030-4
• [Journal Article] The efficacy of dental therapy for an adult case of Henoch-Schönlein Purpura (2012)
  • Author(s): Abe M, Mori Y, Saijo H, Hoshi K, Ohkubo K, Ono T, Takato T
  • Journal Title: Oral Sci Int Volume: 9(2) Pages: 59-62
  • DOI: 10.1016/S1348-8643(12)00027-4
• [Journal Article] Cyclooxygenase-2 activity is important in craniofacial fracture repair (2011)
  • Author(s): Chikazu D, Fujikawa Y, Fujihara H, Suenaga H, Saijo H, Ohkubo K, Ogasawara T, Mori Y, Iino M, Takato T
  • Journal Title: Int. J. Oral Maxillofac. Surg Volume: 40(3) Pages: 322-6
  • DOI: 10.1016/j.ijom.2010.10.011
• [Journal Article] A novel method for designing and fabricating custom-made artificial bones (2011)
  • Author(s): Saijo H, Kanno Y, Mori Y, Suzuki S, Ohkubo K, Chikazu D, Yonehara Y, Chung U, Takato T
  • Journal Title: Int. J. Oral Maxillofac. Surg Volume: 40 Pages: 955-960
  • DOI: 10.1016/j.ijom.2011.03.018
• [Journal Article] Treatment of ankylosed maxillary central incisors by single-tooth dento-osseous osteotomy and alveolar bone distraction (2011)
  • Author(s): Kazumi Ohkubo, Takafumi Susami, Yoshiyuki Mori, Kouhei Nagahama, Naoko Takahashi, Hideto Saijo, Tsuyoshi Takato
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: 111(5) Pages: 561-7
  • DOI: 10.1016/j.tripleo.2010.06.026
• [Presentation] PALATAL INDEX FOR EVALUATION OF THE PALATAL MORPHOLOGY IN PATIENTS WITH UNIATERAL CLEFT LIP AND PALATE (2013)
  • Author(s): Inokuchi T, Susami T, Okayasu M, Uchino N, Uwatoko K, Ohkubo K, Ogasawara T, Saijo H, Mori Y, Takato T
  • Organizer: 12th International Congress on Cleft Lip/Palate and Related Craniofacial Anomalies
  • Place of Presentation: Florida USA ,Hilton Orlando Lake Buena Vista
  • Year and Date: 20130505-10
• [Presentation] TOOTH ALIGNMENT AT THE ALVEOLAR CLEFT SITE AFTER ORTHODONTIC TREATMENT AND THE NEED OF ORTHOGNATHIC SURGERIES (2013)
  • Author(s): Susami T, Uchino N, Ohkubo K, Inokuchi T ,Okayasu M,Uwatoko K, Ogasawara T, Saijo H, Mori Y, Takato T
  • Organizer: 12th International Congress on Cleft Lip/Palate and Related Craniofacial Anomalies
  • Place of Presentation: Florida USA ,Hilton Orlando Lake Buena Vista
  • Year and Date: 20130505-10
• [Presentation] NEED FOR ORTHOGNATHIC SURGERY AND ITS PREDICTION IN EARLY MIXED DENTITION IN PATIENTS WITH BILATERAL CLEFT LIP AND PALATE (2013)
  • Author(s): Okayasu M, Susami T, Inocuchi T, Ohkubo K, Uchino N, Uwatoko K, Ogasawara T, Saijo H, Mori Y, Takato T
  • Organizer: 12th International Congress on Cleft Lip/Palate and Related Craniofacial Anomalies
  • Place of Presentation: Florida USA ,Hilton Orlando Lake Buena Vista
  • Year and Date: 20130505-10
• [Presentation] 東大病院における口唇口蓋裂患者に対する矯正歯科治療(シンポジウムI「こだわりの術式」) (2012)
  • Author(s): 須佐美隆史、大久保和美、井口隆人、岡安麻里、内野夏子、上床喜和子、高戸毅
  • Organizer: 第36回日本口蓋裂学会総会・学術集会
  • Place of Presentation: 国立京都国際会館,京都府京都市
  • Year and Date: 20120524-25
• [Presentation] 二段階下顎骨延長・上顎骨切り術とオトガイ形成術を行った両側性hemifacial microsomiaの1症例 (2011)
  • Author(s): 大久保和美,須佐美隆史,森良之,瀬戸一郎,西條英人,長濱浩平,菅野勇樹,岡安麻里,高戸毅
  • Organizer: 第21回日本頭蓋顎顔面外科学会学術集会
  • Place of Presentation: グラントプリンスホテル高輪,東京
  • Year and Date: 20111124-25
• [Presentation] Maxillary Protraction in Patients with Cleft Lip and Palate : Evaluation after Facial (2011)
  • Author(s): Susami T, Ohkubo K, Nagahama K Takahashi N, Uchino N, Okayasu M, Uwatoko K, Seto I, Saijyo H, Mori Y, Takato T
  • Organizer: 9th European Craniofacial Congress
  • Place of Presentation: Salzburg, Austria, Salzburg Congress
  • Year and Date: 20110914-17
• [Presentation] Outcome of the Surgical-Orthodontic Treatment for Cleft Patients using the Two-stage Maxillary Distraction-Mandibular Setback Osteotomies (2011)
  • Author(s): Susami T, Mori Y, Ohkubo K, Nagahama K, Saijyo H, Takahashi N, Uchino N, Okayasu M, Uwatoko K, Seto I, Takato T
  • Organizer: 9th European Craniofacial Congress
  • Place of Presentation: Salzburg, Austria, Salzburg Congress
  • Year and Date: 20110914-17
• [Presentation] Implant-type tissue-engineered cartilage applied for the nasal correction of the cleft lip and palate patients (2011)
  • Author(s): Takato T, Mori Y, Saijo H, Kanno Y, Kurabayashi K, Sugiyama M, Susami T, Ohkubo K, Okayasu M, Uchino N, Nishizawa S, Fujihara Y, Hoshi K
  • Organizer: 9th European Craniofacial Congress
  • Place of Presentation: Salzburg, Austria, Salzburg Congress
  • Year and Date: 20110914-17
• [Presentation] Implant-Type Tissue-Engineered Cartilage Applied For The Correction Of The Cleft Lip-Nose Patients (2011)
  • Author(s): Takato T, Mori Y, Saijo H, Seto I, Kanno Y, Kurabayashi K, Sugiyama M, Susami T, Ohkubo K, Okayasu M, Uchino N, Nishizawa S, Fujihara Y, Hoshi K
  • Organizer: in Tissue Engineering and Regenerative Medicine International Society Asia Pacific Meeting
  • Place of Presentation: Singapore, Waterfront Conference Centre
  • Year and Date: 20110803-05
• [Book] 医学部附属病院における矯正歯科クインテッセンス『臨床家のための矯正イヤーブック'11』 (2011)
  • Author(s): 須佐美隆史、大久保和美、長濱浩平、高戸毅
  • Total Pages: 41-44
  • Publisher: 伊藤学而/中島榮一郎編集,クインテッセンス出版,東京
• [Remarks]
  • URL: http://plaza.umin.ac.jp/~oralsurg/archive.html