2012 Fiscal Year Final Research Report
Molecular mechanism and quantitative analysis of microRNA-mediated gene silencing
| Project/Area Number |
23651200
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UI-TEI Kumiko 東京大学, 大学院・理学系研究科, 准教授 (50213327)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHI Kenji 東京大学, 大学院・理学系研究科, 助教 (50466801)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 機能性 RNA / miRNA silencing |
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| Research Abstract |
In this study, we performed studies as follows: (1) When exogenous miRNAs (exo-miRNAs) are introduced into cells, endogenous miRNAs (endo-miRNAs) in the RISC may be replaced with exo-miRNAs. We examined the fluctuation of target gene expression of endo-miRNAs response to the introduction of an exo-miRNA using microarray. (2) We identified the crucial factors that determine the efficacy of miRNA-mediated gene silencing, and successfully mathematized the silencing efficacy using values reflecting base-pairing stabilities in miRNA duplex and seed-target duplex.
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[Journal Article] IFPA meeting 2012 workshop report I:Comparative placentation and animal models, advanced techniques in placental histopathology,human pluripotent stem cells as a model for trophoblast differentiation2013
Author(s)
Ackerman WE 4th, Carter AM, De Mestre AM, Golos TG, Jeschke U, Kusakabe K, Laurent LC, Parast MM, Roberts RM, Robinson JM, Rutherford J, Soma H, Takizawa T, Ui-Tei K,Las GE.
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Journal Title
Placenta
Volume: vol.34
Pages: S3-5
Peer Reviewed
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