Molecular ecological analyses of nitrogen-fixing bacterial consortia in rice rhizospere and plant

2011 Fiscal Year Final Research Report

• Project/Area Number: 23658072
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo
• Principal Investigator: HIDAKA Makoto 東京大学, 大学院・農学生命科学研究科, 准教授 (50183918)
• Project Period (FY): 2011
• Keywords: 窒素固定細菌 / 微生物間相互作用 / 生物窒素肥料

Research Abstract

Relationship between nitrogen-fixing activity of Klebsiella oxytoca R16, a diazotroph living in rice rhizosphere, and its bacterial interaction in rhizosphere flora was analyzed. Strain R16 was cultivated together with 9 strains of non-diazotroph isolated form paddy soil. Pseudomonas sp. RKF06 enhanced the nitrogen-fixing activity of R16 significantly. In inoculation assay of R16 into the rhizosphere of rice, R16 showed higher nitrogen-fixing activity when strain RKF06 was inoculated simultaneously. This finding will be a new manner to take advantage of diazotrophs.