Screening of posttranslationally modified peptide containing isoprenylated tryptophane residue.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23658098
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Chubu University
• Principal Investigator: OKADA Masahiro 中部大学, 応用生物学部, 講師 (40377792)
• Project Period (FY): 2011 – 2012
• Keywords: 翻訳後修飾 / イソプレニル化 / トリプトファン / 枯草菌 / クオラムセンシング

Research Abstract

In order to confirm that isoprenylation of tryptophan residues is a universal posttranslational modification, I developed a cell-free assay system by which the tryptophan residue in the ComX pheromone precursor can be isoprenylated by themodifying enzyme, ComQ, with isoprenyl pyrophosphate and magnesium ion.

Research Products

• [Journal Article] Lack of the consensus sequence necessary for tryptophan prenylation in the ComX pheromone precursor. (2012)
  • Author(s): F. Tsuji, A. Ishihara, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
  • Journal Title: Biotechnol. Bio chem. Volume: 76 Pages: 1492-1496
  • DOI: DOI:doi.org/ 10.1271/ bbb.120206
  • Peer Reviewed
• [Journal Article] Creation of pure nanodrugs and their anticancer properties. (2012)
  • Author(s): H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, and M.Hashida.
  • Journal Title: Angew. Chem. Int. Ed Volume: 51 Pages: 10315-10318
  • DOI: DOI:doi.org/ 10.1002/ anie.201204596
  • Peer Reviewed
• [Journal Article] Geranyl modification on the tryptophan residue of ComXRO-E-2 pheromone by a cell-free system. (2012)
  • Author(s): F. Tsuji, A. Ishihara, K. Kurata, A. Nakagawa, M. Okada, S. Kitamura, K. Kanamaru, Y. Masuda, K. Murakami, K. Irie, and Y. Sakagami.
  • Journal Title: FEBS Lett Volume: 586 Pages: 174-179
  • DOI: DOI:doi.org/10.1016/j.febslet.2011.12.012
  • Peer Reviewed
• [Journal Article] The geranyl-modified tryptophan residue is crucial for ComXRO-E-2 pheromone biological activity. (2011)
  • Author(s): F. Tsuji, K. Kobayashi, M. Okada, H. Yamaguchi, M. Ojika and Y. Sakagami.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 21 Pages: 4041-4044
  • DOI: DOI:doi.org/10.1016/j.febslet.2011.12.012
  • Peer Reviewed
• [Journal Article] Post-translational isoprenylation of tryptophan. (2011)
  • Author(s): M. Okada.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 75 Pages: 1413-1417
  • DOI: DOI:doi.org/10.1271/bbb.110087
  • Peer Reviewed
• [Presentation] Peptide isoprenylation (2012)
  • Author(s): M. Okada.
  • Organizer: International Symposium on Natural Products Chemistry and Chemical Biology 2012 (招待講演)
  • Place of Presentation: Hangzhou, China
  • Year and Date: 20121123-26
• [Presentation] 翻訳後修飾によるトリプト ファンのイソプレニル化 (2011)
  • Author(s): 岡田 正弘.
  • Organizer: 第三回有機「ものつくり」化学研究会
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2011-08-27
• [Book] Posttranslational isoprenylation of tryptophan residues in Bacillus subtilis. The Enzymes (2011)
  • Author(s): M. Okada, F. Tsuji and Y.Sakagami.
  • Total Pages: 183-194
  • Publisher: Academic Press, San Diego, CA.